| VALIDATION OF QUANTITATIVE ELISAs FOR MEASURING ANTI-YERSINIA PESTIS F1 AND V ANTIBODY CONCENTRATIONS IN NONHUMAN PRIMATE SERA. | |
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MedLine Citation:
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PMID: 22181824 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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This study systematically validated two quantitative enzyme-linked immunosorbent assays (ELISAs) for determining Yersinia pestis anti-F1 or anti-V IgG concentration in cynomolgus macaque sera. The results demonstrated that these ELISAs are reliable, reproducible, and suitable for their intended use to measure both anti-F1 and anti-V IgG in monkey sera following vaccination with a heterologous recombinant fusion F1-V protein (rF1-V). Statistical analysis demonstrated assay precision, accuracy, specificity, linearity/dilutional linearity, and robustness for both assays. The quantitative ranges of standard curves were defined as 40-700 ng/mLfor both anti-F1 and anti-V IgG. Either serological assay could be used to determine potency of F1/V antigen-based vaccines in surrogate clinical studies or to define correlates of protective immunity against plague under the Food and Drug Administration's (FDA) two-animal rule. |
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Authors:
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C Y Lindsey; B S Powell; C R Bolt; J E Brown; J J Adamovicz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunoassay & immunochemistry Volume: 33 ISSN: 1532-4230 ISO Abbreviation: J Immunoassay Immunochem Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2011-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100963688 Medline TA: J Immunoassay Immunochem Country: England |
Other Details:
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Languages: eng Pagination: 91-113 Citation Subset: IM |
Affiliation:
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a United States Army Medical Research Institute of Infectious Diseases , Fort Detrick , Maryland , USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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