| V108M mutant of pharaonis phoborhodopsin: substitution caused no absorption change but affected its M-state. | |
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MedLine Citation:
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PMID: 9685733 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Crystallographic data reveal that Met-118 in bacteriorhodopsin (bR) contacts directly with the C9 methyl group of retinal, and Khorana et al. [J. Biol. Chem. 268, 20305-20311 (1993)] suggest that this contact may regulate the absorption maximum (lambdamax). We have replaced the amino acid (Val-108) corresponding to Met-118 of bR by methionine in pharaonis phoborhodopsin (ppR), whose lambdamax is ca. 500 nm, while those of other bacterial rhodopsins such as bR, halorhodopsin, and sensory rhodopsin are red-shifted by 60-90 nm. By flash-photolysis measurement, we could not recognize a large spectral red-shift of the V108M mutant. On the other hand, the decay of ppRM (M-intermediate) of the mutant was approximately three times as fast as that of wild-type, and an M-like intermediate (M') whose lambdamax is blue-shifted by 60 nm from that of M became appreciable. The replacement abolished the shoulder of the ppRM spectrum. From these findings, we infer that the distance between the retinal and the 108-position in ppR is relatively long, and that in the M-state this distance is shortened. |
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Authors:
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K Shimono; M Iwamoto; M Sumi; N Kamo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 124 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-11-13 Completed Date: 1998-11-13 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 404-9 Citation Subset: IM |
Affiliation:
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Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060-0812, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Archaeal Proteins* Bacteriorhodopsins / chemistry*, genetics Carotenoids* Escherichia coli Halorhodopsins* Methionine / chemistry Molecular Sequence Data Mutagenesis, Site-Directed Sensory Rhodopsins* Sequence Homology, Amino Acid Spectrum Analysis Valine / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Archaeal Proteins; 0/Halorhodopsins; 0/Sensory Rhodopsins; 0/sensory rhodopsin II protein, archaeal; 36-88-4/Carotenoids; 53026-44-1/Bacteriorhodopsins; 63-68-3/Methionine; 7004-03-7/Valine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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