Document Detail

V-fusion: a convenient, nontoxic method for cell fusion.
MedLine Citation:
PMID:  20964635     Owner:  NLM     Status:  In-Data-Review    
Cell-to-cell fusion (cell fusion) is a fundamental biological process that also has been used as a versatile experimental tool to dissect a variety of cellular mechanisms, including the consequences of cell fusion itself, and to produce cells with desired properties, such as hybridomas and reprogrammed progenitors. However, current methods of cell fusion are not satisfactory because of their toxicity, inefficiency, or lack of flexibility. We describe a simple, versatile, scalable, and nontoxic approach that we call V-fusion, as it is based on the ability of the vesicular stomatitis virus G protein (VSV-G), a viral fusogen of broad tropism, to become rapidly and reversibly activated. We suggest that this approach will benefit a broad array of studies that investigate consequences of cell fusion or use cell fusion as an experimental tool.
Amy Gottesman; Joseph Milazzo; Yuri Lazebnik
Related Documents :
14645125 - Death is the major fate of medial edge epithelial cells and the cause of basal lamina d...
4340165 - Cell cycle changes and the ability of cells to undergo virus-induced fusion.
17166005 - Controlled assembly of hybrid bulk-heterojunction solar cells by sequential deposition.
611005 - Complementation between senescent human diploid cells and a thymidine kinase-deficient ...
2981885 - Site-specific maturation of enveloped viruses in l cells treated with cytochalasin b.
913975 - Proliferation rate and transit time of mucosal cells in small intestine of the diabetic...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioTechniques     Volume:  49     ISSN:  1940-9818     ISO Abbreviation:  BioTechniques     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  England    
Other Details:
Languages:  eng     Pagination:  747-50     Citation Subset:  IM    
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11768, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A nerve clamp electrode design for indirect stimulation of skeletal muscle.
Next Document:  Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep se...