Document Detail


V-fusion: a convenient, nontoxic method for cell fusion.
MedLine Citation:
PMID:  20964635     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cell-to-cell fusion (cell fusion) is a fundamental biological process that also has been used as a versatile experimental tool to dissect a variety of cellular mechanisms, including the consequences of cell fusion itself, and to produce cells with desired properties, such as hybridomas and reprogrammed progenitors. However, current methods of cell fusion are not satisfactory because of their toxicity, inefficiency, or lack of flexibility. We describe a simple, versatile, scalable, and nontoxic approach that we call V-fusion, as it is based on the ability of the vesicular stomatitis virus G protein (VSV-G), a viral fusogen of broad tropism, to become rapidly and reversibly activated. We suggest that this approach will benefit a broad array of studies that investigate consequences of cell fusion or use cell fusion as an experimental tool.
Authors:
Amy Gottesman; Joseph Milazzo; Yuri Lazebnik
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioTechniques     Volume:  49     ISSN:  1940-9818     ISO Abbreviation:  BioTechniques     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  England    
Other Details:
Languages:  eng     Pagination:  747-50     Citation Subset:  IM    
Affiliation:
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11768, USA.
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