Document Detail


V-ATPase upregulation during early pregnancy: A possible link to establishment of an inflammatory response during preimplantation period of pregnancy.
MedLine Citation:
PMID:  22454532     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
During pregnancy there is a necessary early preimplantation inflammatory stage, followed by a post implantation anti-inflammatory stage. Thus there is a biphasic "immune response" observed during the course of pregnancy. We provide the evidence that capacitation of sperm induced the expression of a2 isoform of V-ATPase (ATP6V0A2 referred as a2V), leukemia inhibitory factor (Lif), Il1b, and Tnf in the sperm. Capacitated sperm also released cleaved N-terminal domain of a2V-ATPase (a2NTD) which upregulates the gene expression of Lif, Il1b, Tnf and monocyte chemotactic protein-1 (Mcp1) in the uterus. Unfertilized eggs had low a2V expression but after fertilization, the expression of a2V increased in zygotes. This increased level of a2V expression was maintained in preimplantation embryos. Seminal plasma was necessary for upregulation of a2V expression in preimplantation embryos, since mating with seminal vesicle deficient-males failed to elicit an increase of a2V expression in preimplantation embryos. The infiltration of macrophages into the uterus was significantly increased after insemination of both sperm and seminal plasma during preimplantation period of pregnancy. This dynamic infiltration into the uterus corresponded with the uterine a2V expression through the induction of Mcp1 expression. Furthermore, the polarization ratio of M1/M2 (pro-inflammatory/anti-inflammatory) macrophages in the uterus fluctuated from a ratio of 1.60 (Day 1) to 1.45 (Day 4) when female mice were inseminated with both sperm and seminal plasma. This data provide evidence that exposure to semen may initiate an inflammatory milieu by inducing a2V and cytokine/chemokine expression which triggers the influx of macrophages into preimplantation uterus during the onset of pregnancy and ultimately leads to successful pregnancy outcome.
Authors:
Mukesh Kumar Jaiswal; Timothy M Mallers; Benjamin Larsen; Joanne Kwak-Kim; Gerard Chaouat; Alice Gilman-Sachs; Kenneth D Beaman
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-27
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  -     ISSN:  1741-7899     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
M Jaiswal, Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science, North Chicago, United States.
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