Document Detail


Utilizing confocal microscopy to measure refractive index of articular cartilage.
MedLine Citation:
PMID:  23140377     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This study proposes a method for measuring the refractive index of articular cartilage within a thin and small specimen slice. The cartilage specimen, with a thickness of about 50 μm, was put next to a thin film of immersion oil of similar thickness. Both the articular cartilage and immersion oil were scanned along the depth direction using a confocal microscope. The refractive index mismatch between the cartilage and the immersion oil induced a slight axial deformation in the confocal images of the cartilage specimen that was accurately measured by a subpixel edge-detection-based technique. A theoretical model was built to quantify the focal shift of confocal microscopy caused by the refractive index mismatch. With the quantitative deformations of cartilage images and the quantified function of focal shift, the refractive index of articular cartilage was accurately interpolated. At 561 nm, 0.1 MPa and 20 °C, the overall refractive index of the six cartilage plugs was 1.3975 ± 0.0156. The overall coefficient of variation of all cartilage specimens was 0.68%, which indicated the high repeatability of our method. The verification experiments using distilled water showed a minimal relative error of 0.02%.
Authors:
K Wang; J Wu; R E Day; T B Kirk
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  248     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  281-91     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors Journal of Microscopy © 2012 Royal Microscopical Society.
Affiliation:
School of Mechanical and Chemical Engineering, University of Western Australia, Perth, WA, Australia Department of Mechanical Engineering, Curtin University, Perth, WA, Australia Department of Medical Engineering and Physics, Royal Perth Hospital, Perth, WA, Australia.
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