| Utilizing acid pretreatment and electrospinning to improve biocompatibility of poly(glycolic acid) for tissue engineering. | |
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MedLine Citation:
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PMID: 15368238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly(glycolic acid) (PGA) has a long history as a bioresorbable polymer. Its biocompatibility is widely accepted, yet PGA is often rejected as a soft-tissue scaffold because of fibrous encapsulation. The goal of this study was to improve the soft-tissue biocompatibility of PGA by producing scaffolds composed of small-diameter fibers through electrospinning and subjecting these scaffolds to a concentrated hydrochloric acid (HCL) pretreatment. The theory is that small-diameter fibers will elicit a reduced immune response and HCl treatment will improve cellular interactions. Scaffolds were characterized in terms of fiber diameter and pore area via image-analysis software. Biocompatibility was assessed through a WST-1 cell-proliferation assay (in vitro) with the use of rat cardiac fibroblasts and rat intramuscular implantations (in vivo). Fibers produced ranged in diameter from 0.22 to 0.88 microm with pore areas from 1.84 to 13.22 microm(2). The untreated scaffold composed of 0.88-microm fibers was encapsulated in vivo and supported the lowest rates of cell proliferation. On the contrary, the acid pretreated scaffold with 0.22-microm fibers was incorporated into the surrounding tissue and exhibited proliferation rates that exceeded the control populations on tissue-culture plastic. In conclusion, this study has shown the ability to improve the biocompatibility of PGA through acid pretreatment of scaffolds comprised of submicron fiber diameters. |
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Authors:
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Eugene D Boland; Todd A Telemeco; David G Simpson; Gary E Wnek; Gary L Bowlin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part B, Applied biomaterials Volume: 71 ISSN: 1552-4973 ISO Abbreviation: J. Biomed. Mater. Res. Part B Appl. Biomater. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-15 Completed Date: 2005-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101234238 Medline TA: J Biomed Mater Res B Appl Biomater Country: United States |
Other Details:
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Languages: eng Pagination: 144-52 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia 23298, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials* Male Materials Testing Microscopy, Electron, Scanning Polyglycolic Acid* / chemistry Prostheses and Implants Rats Tissue Engineering / methods* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 26009-03-0/Polyglycolic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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