Document Detail

Utilization of tannery fleshings: Optimization of conditions for fermenting delimed tannery fleshings using Enterococcus faecium HAB01 by response surface methodology.
MedLine Citation:
PMID:  19892548     Owner:  NLM     Status:  MEDLINE    
Conditions for fermentation of delimed tannery fleshings--to obtain higher degree of protein hydrolysis and reasonably better antioxidant activity--using Enterococcus faecium HAB01 (GenBank #FJ418568) were optimized. Three independent variables--viz., inoculum level (X1), glucose level (X2) and fermentation time (X3)--were optimized using response surface method considering degree of hydrolysis (DH; %) and total titrable acidity (TTA) as response variables. The optimized conditions were found to be 12.5% (v/w) inoculum, 17.5% (w/w) glucose and 96h of fermentation at 37+/-1 degrees C to obtain a maximum DH%. The usefulness of the predicted model was further validated by considering random combinations of the independent factors. The chemical score of the hydrolysate revealed an excess amount of essential amino acids, viz., arginine and leucine compared to reference protein. The liquor portion had relatively high antioxidant activities, indicating its potential for use as a high value feed ingredient.
Amit Kumar Rai; Thiyam General; N Bhaskar; P V Suresh; P Z Sakhare; P M Halami; Lalitha R Gowda; N S Mahendrakar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-04
Journal Detail:
Title:  Bioresource technology     Volume:  101     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-10-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1885-91     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Elsevier Ltd. All rights reserved.
Department of Meat, Fish and Poultry Technology, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore 570 020, Karnataka, India.
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MeSH Terms
Antioxidants / chemistry
Arginine / chemistry
Biotechnology / methods
Biphenyl Compounds
Enterococcus faecium / metabolism*
Free Radicals
Glucose / chemistry
Hydrogen-Ion Concentration
Industrial Waste*
Leucine / chemistry
Surface Properties
Reg. No./Substance:
0/Antioxidants; 0/Biphenyl Compounds; 0/Free Radicals; 0/Industrial Waste; 0/Picrates; 1898-66-4/2,2-diphenyl-1-picrylhydrazyl; 50-99-7/Glucose; 61-90-5/Leucine; 74-79-3/Arginine

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