Document Detail


Utilization of porcine pancreatic phospholipase A2 for the preparation of a marine fish oil enriched in (n - 3) polyunsaturated fatty acids.
MedLine Citation:
PMID:  3828085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and rapid method is described for the preparation of a marine oil fraction highly enriched in (n - 3) polyunsaturated fatty acids. Cod roe, containing lipid up to 15% of its dry weight, approximately 70% of which is phospholipid, was the starting material. Incubation of a concentrated aqueous extract of the roe with porcine pancreatic phospholipase A2 (EC 3.1.1.4) was the key step in the procedure. Extraction of the freeze-dried reaction product with diethyl ether containing formic acid produced an oil in a yield of 1.0 g/100 g wet wt of starting roe. The oil contained over 95% as free fatty acids, with 20:5 (n - 3) and 22:6 (n - 3) accounting for up to 24 and 40%, respectively, of the total free fatty acids. The therapeutic use of the oil is mentioned.
Authors:
D R Tocher; A Webster; J R Sargent
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  8     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1987-05-04     Completed Date:  1987-05-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  83-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Fatty Acids, Unsaturated / isolation & purification*
Fish Oils / analysis*
Freeze Drying
Hydrolysis
Pancreas / enzymology*
Phospholipases*
Phospholipases A*
Phospholipases A2
Swine
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Fish Oils; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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