| Utilization of porcine pancreatic phospholipase A2 for the preparation of a marine fish oil enriched in (n - 3) polyunsaturated fatty acids. | |
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MedLine Citation:
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PMID: 3828085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and rapid method is described for the preparation of a marine oil fraction highly enriched in (n - 3) polyunsaturated fatty acids. Cod roe, containing lipid up to 15% of its dry weight, approximately 70% of which is phospholipid, was the starting material. Incubation of a concentrated aqueous extract of the roe with porcine pancreatic phospholipase A2 (EC 3.1.1.4) was the key step in the procedure. Extraction of the freeze-dried reaction product with diethyl ether containing formic acid produced an oil in a yield of 1.0 g/100 g wet wt of starting roe. The oil contained over 95% as free fatty acids, with 20:5 (n - 3) and 22:6 (n - 3) accounting for up to 24 and 40%, respectively, of the total free fatty acids. The therapeutic use of the oil is mentioned. |
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Authors:
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D R Tocher; A Webster; J R Sargent |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and applied biochemistry Volume: 8 ISSN: 0885-4513 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 1986 Feb |
Date Detail:
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Created Date: 1987-05-04 Completed Date: 1987-05-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 83-95 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fatty Acids, Unsaturated / isolation & purification* Fish Oils / analysis* Freeze Drying Hydrolysis Pancreas / enzymology* Phospholipases* Phospholipases A* Phospholipases A2 Swine |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Fish Oils; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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