| Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. | |
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MedLine Citation:
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PMID: 15870323 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have isolated from plant surfaces several bacteria with the ability to catabolize indole-3-acetic acid (IAA). One of them, isolate 1290, was able to utilize IAA as a sole source of carbon, nitrogen, and energy. The strain was identified by its 16S rRNA sequence as Pseudomonas putida. Activity of the enzyme catechol 1,2-dioxygenase was induced during growth on IAA, suggesting that catechol is an intermediate of the IAA catabolic pathway. This was in agreement with the observation that the oxygen uptake by IAA-grown P. putida 1290 cells was elevated in response to the addition of catechol. The inability of a catR mutant of P. putida 1290 to grow at the expense of IAA also suggests a central role for catechol as an intermediate in IAA metabolism. Besides being able to destroy IAA, strain 1290 was also capable of producing IAA in media supplemented with tryptophan. In root elongation assays, P. putida strain 1290 completely abolished the inhibitory effect of exogenous IAA on the elongation of radish roots. In fact, coinoculation of roots with P. putida 1290 and 1 mM concentration of IAA had a positive effect on root development. In coinoculation experiments on radish roots, strain 1290 was only partially able to alleviate the inhibitory effect of bacteria that in culture overproduce IAA. Our findings imply a biological role for strain 1290 as a sink or recycler of IAA in its association with plants and plant-associated bacteria. |
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Authors:
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Johan H J Leveau; Steven E Lindow |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 71 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-05-04 Completed Date: 2005-06-15 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2365-71 Citation Subset: IM |
Affiliation:
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Netherlands Institute of Ecology (NIOO-KNAW), Centre for Terrestrial Ecology, Boterhoeksestraat 48, 6666 GA Heteren, The Netherlands. j.leveau@nioo.knaw.nl |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY491973 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Culture Media Indoleacetic Acids / metabolism* Molecular Sequence Data Phylogeny Plant Roots / growth & development Pseudomonas putida / growth & development*, metabolism RNA, Ribosomal, 16S / genetics |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Indoleacetic Acids; 0/RNA, Ribosomal, 16S; 87-51-4/indoleacetic acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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