Document Detail


Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290.
MedLine Citation:
PMID:  15870323     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated from plant surfaces several bacteria with the ability to catabolize indole-3-acetic acid (IAA). One of them, isolate 1290, was able to utilize IAA as a sole source of carbon, nitrogen, and energy. The strain was identified by its 16S rRNA sequence as Pseudomonas putida. Activity of the enzyme catechol 1,2-dioxygenase was induced during growth on IAA, suggesting that catechol is an intermediate of the IAA catabolic pathway. This was in agreement with the observation that the oxygen uptake by IAA-grown P. putida 1290 cells was elevated in response to the addition of catechol. The inability of a catR mutant of P. putida 1290 to grow at the expense of IAA also suggests a central role for catechol as an intermediate in IAA metabolism. Besides being able to destroy IAA, strain 1290 was also capable of producing IAA in media supplemented with tryptophan. In root elongation assays, P. putida strain 1290 completely abolished the inhibitory effect of exogenous IAA on the elongation of radish roots. In fact, coinoculation of roots with P. putida 1290 and 1 mM concentration of IAA had a positive effect on root development. In coinoculation experiments on radish roots, strain 1290 was only partially able to alleviate the inhibitory effect of bacteria that in culture overproduce IAA. Our findings imply a biological role for strain 1290 as a sink or recycler of IAA in its association with plants and plant-associated bacteria.
Authors:
Johan H J Leveau; Steven E Lindow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  71     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-04     Completed Date:  2005-06-15     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2365-71     Citation Subset:  IM    
Affiliation:
Netherlands Institute of Ecology (NIOO-KNAW), Centre for Terrestrial Ecology, Boterhoeksestraat 48, 6666 GA Heteren, The Netherlands. j.leveau@nioo.knaw.nl
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY491973
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Culture Media
Indoleacetic Acids / metabolism*
Molecular Sequence Data
Phylogeny
Plant Roots / growth & development
Pseudomonas putida / growth & development*,  metabolism
RNA, Ribosomal, 16S / genetics
Chemical
Reg. No./Substance:
0/Culture Media; 0/Indoleacetic Acids; 0/RNA, Ribosomal, 16S; 6U1S09C61L/indoleacetic acid
Comments/Corrections

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