Document Detail

Utilization of ornithine and arginine as specific precursors of clavulanic acid.
MedLine Citation:
PMID:  2877616     Owner:  NLM     Status:  MEDLINE    
Ornithine and arginine (5 to 20 mM), but not glutamic acid or proline, exerted a concentration-dependent stimulatory effect on the biosynthesis of clavulanic acid in both resting-cell cultures and long-term fermentations of Streptomyces clavuligerus. Ornithine strongly inhibited cephamycin biosynthesis in the same strain. [1-14C]-, [5-14C]-, or [U-14 C] ornithine was efficiently incorporated into clavulanic acid, whereas the incorporation of uniformly labeled glutamic acid was very poor. [U-14C] citrulline were not incorporated at all. Mutant nca-1, a strain that is blocked in clavulanic acid biosynthesis, did not incorporate arginine into clavulanic acid. S. clavuligerus showed arginase activity, converting arginine into ornithine, but not amidinotransferase activity. Both arginase activity and clavulanic acid formation were enhanced simultaneously by supplementing the production medium with 10 mM arginine.
J Romero; P Liras; J F Martín
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  52     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-12-18     Completed Date:  1986-12-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  892-7     Citation Subset:  IM    
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MeSH Terms
Arginine / metabolism*
Cephamycins / biosynthesis
Clavulanic Acids / biosynthesis*
Glutamates / metabolism
Glutamic Acid
Ornithine / metabolism*,  pharmacology
Proline / metabolism
Streptomyces / metabolism*
Reg. No./Substance:
0/Cephamycins; 0/Clavulanic Acids; 0/Glutamates; 147-85-3/Proline; 56-86-0/Glutamic Acid; 7006-33-9/Ornithine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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