| Utilization of the nitrate reductase enzymatic pathway to reduce enteric pathogens in chickens. | |
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MedLine Citation:
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PMID: 15554062 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous reports have shown that some bacteria, including Salmonella, use a dissimilatory nitrate reductase enzyme pathway (NREP) in anaerobic environments. This enzyme reduces nitrate to nitrite and has been shown to cometabolize chlorate to cytotoxic chlorite. The present investigations were performed to evaluate the susceptibility of a competitive exclusion culture (CE) to the experimental chlorate product (ECP). A commercially available CE product was evaluated for its nitrate reductase activity and therefore its chlorate sensitivity. Individual isolates (in triplicate) were cultured in 10 mL of Viande Levure broth containing 5 mM sodium nitrate or 10 mM sodium chlorate. Bacterial growth (optical density at 625 nm) was measured and 1-mL aliquots were removed concurrently for colorimetric determination of nitrate content at 0, 3, 6, and 24 h. Of the 15 different facultative strains, 11 had slight NREP utilization, 3 had moderate NREP utilization, and the remainder were NREP negative (with slight and moderate NREP utilization: >0.1 to <1.0 mM and >1.0 mM nitrate used within 6 h, respectively). Of the obligate anaerobes evaluated, 3 had slight NREP utilization and the remainder were NREP negative. In vivo studies utilizing both products (CE and ECP) in a horizontal transmission challenge model (seeders + contacts) showed significant reductions in Salmonella from 5.37 to 1.76 log10 cfu/g and 3.94 to 0.07 log10 cfu/g, respectively. The combined effect of the CE culture and an ECP are effective in killing these food-borne pathogens. |
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Authors:
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J L McReynolds; J A Byrd; R W Moore; R C Anderson; T L Poole; T S Edrington; L F Kubena; D J Nisbet |
Publication Detail:
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Type: Clinical Trial; Controlled Clinical Trial; Journal Article |
Journal Detail:
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Title: Poultry science Volume: 83 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-11-19 Completed Date: 2004-12-08 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1857-60 Citation Subset: IM |
Affiliation:
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USDA-ARS, Southern Plains Agricultural Research Center, College Station, Texas 77845, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Husbandry
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methods Animals Anti-Infective Agents Chickens / microbiology* Chlorates / metabolism* Feces / microbiology Intestines / microbiology Nitrate Reductase Nitrate Reductases / metabolism* Organic Chemicals Salmonella / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents; 0/Chlorates; 0/Organic Chemicals; 0/preempt; EC 1.7.-/Nitrate Reductases; EC 1.7.99.4/Nitrate Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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