Document Detail

Utilization of the nitrate reductase enzymatic pathway to reduce enteric pathogens in chickens.
MedLine Citation:
PMID:  15554062     Owner:  NLM     Status:  MEDLINE    
Previous reports have shown that some bacteria, including Salmonella, use a dissimilatory nitrate reductase enzyme pathway (NREP) in anaerobic environments. This enzyme reduces nitrate to nitrite and has been shown to cometabolize chlorate to cytotoxic chlorite. The present investigations were performed to evaluate the susceptibility of a competitive exclusion culture (CE) to the experimental chlorate product (ECP). A commercially available CE product was evaluated for its nitrate reductase activity and therefore its chlorate sensitivity. Individual isolates (in triplicate) were cultured in 10 mL of Viande Levure broth containing 5 mM sodium nitrate or 10 mM sodium chlorate. Bacterial growth (optical density at 625 nm) was measured and 1-mL aliquots were removed concurrently for colorimetric determination of nitrate content at 0, 3, 6, and 24 h. Of the 15 different facultative strains, 11 had slight NREP utilization, 3 had moderate NREP utilization, and the remainder were NREP negative (with slight and moderate NREP utilization: >0.1 to <1.0 mM and >1.0 mM nitrate used within 6 h, respectively). Of the obligate anaerobes evaluated, 3 had slight NREP utilization and the remainder were NREP negative. In vivo studies utilizing both products (CE and ECP) in a horizontal transmission challenge model (seeders + contacts) showed significant reductions in Salmonella from 5.37 to 1.76 log10 cfu/g and 3.94 to 0.07 log10 cfu/g, respectively. The combined effect of the CE culture and an ECP are effective in killing these food-borne pathogens.
J L McReynolds; J A Byrd; R W Moore; R C Anderson; T L Poole; T S Edrington; L F Kubena; D J Nisbet
Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article    
Journal Detail:
Title:  Poultry science     Volume:  83     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-19     Completed Date:  2004-12-08     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1857-60     Citation Subset:  IM    
USDA-ARS, Southern Plains Agricultural Research Center, College Station, Texas 77845, USA.
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MeSH Terms
Animal Husbandry / methods
Anti-Infective Agents
Chickens / microbiology*
Chlorates / metabolism*
Feces / microbiology
Intestines / microbiology
Nitrate Reductase
Nitrate Reductases / metabolism*
Organic Chemicals
Salmonella / drug effects*
Reg. No./Substance:
0/Anti-Infective Agents; 0/Chlorates; 0/Organic Chemicals; 0/preempt; EC 1.7.-/Nitrate Reductases; EC Reductase

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