| Utilization of microarray platforms in clinical practice: an insight on the preparation and amplification of nucleic acids from frozen and fixed tissues. | |
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MedLine Citation:
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PMID: 18220228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The last decade has witnessed an impressive upsurge in the utilization of microarray platforms for biomedical research. However, the application of this emerging technology in medical practice lagged behind. This lag is understandable because there are specific issues pertaining to the utilization of clinical samples, which has to be rigorously addressed and overcome before microarrays enter mainstream medical practice. Such issues include cost, ethics, the complexity and heterogeneity of human tissue architecture, and their corresponding diseases, the type of tissues to be used, nucleic acids amplification, and experimental variability. As microarrays enter, albeit cautiously, the frontline of clinical practice, investigators and clinicians require to set up protocols that address these issues. This chapter decribes the methods used for nucleic acids preparation from frozen and formalin-fixed paraffin-embedded human tissues using macro-and microdissection and show their suitability for use in microarray experiments. |
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Authors:
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Fahd Al-Mulla |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 382 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2008-01-28 Completed Date: 2008-03-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 115-36 Citation Subset: IM |
Affiliation:
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Department of Pathology, Faculty of Medicine, Kuwait University, Safat. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Neoplasm
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genetics Gene Expression Profiling / methods* Humans Microdissection Neoplasms / genetics*, pathology Nucleic Acid Amplification Techniques* Oligonucleotide Array Sequence Analysis / methods* Paraffin Embedding Polymerase Chain Reaction / methods RNA, Neoplasm / genetics Tissue Fixation* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/RNA, Neoplasm |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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