Document Detail


Utilization of endogenous lipid by the isolated perfused rat heart.
MedLine Citation:
PMID:  6049403     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The lipids of the rat heart have been studied with regard to amount, classes present and fatty acid composition of free fatty acids, triglycerides and phospholipids. Myocardial lipid contained 300mumoles of total fatty acid/g. dry wt. of which only 2-4mumoles were free; the remainder was esterified, chiefly as phospholipid. Neutral esters, of which triglyceride was the principal form, made up 15% of the total fatty acids. 2. When normal hearts were perfused with a nutrient-free medium until exhaustion, the triglyceride concentration declined from 43 to 13mumoles/g. dry wt. The content of phospholipids, partial glycerides and cholesteryl esters did not change. When the lipids of the rat heart were labelled with [1-(14)C]palmitate before perfusion with non-nutrient medium, radioactivity disappeared from the triglyceride, diglyceride and free fatty acid fractions, but not from the phospholipid or other ester classes. 3. These experiments support the view that only a small fraction of the total cardiac lipid, principally triglycerides and to a smaller extent diglycerides, is available as a source of fuel in the absence of exogenous substrate.
Authors:
R E Olson; R J Hoeschen
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  103     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1967 Jun 
Date Detail:
Created Date:  1967-12-14     Completed Date:  1967-12-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  796-801     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Isotopes
Cholesterol / analysis
Chromatography, Gas
Fatty Acids, Nonesterified / analysis
Glycerides / analysis
Lipid Metabolism*
Lipids / analysis
Male
Myocardium / analysis,  metabolism*
Palmitic Acids / metabolism
Perfusion
Phospholipids / analysis
Rats
Triglycerides / analysis
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids, Nonesterified; 0/Glycerides; 0/Lipids; 0/Palmitic Acids; 0/Phospholipids; 0/Triglycerides; 57-88-5/Cholesterol
Comments/Corrections

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