Document Detail


Utilization of ascites plasma very low density lipoprotein triglycerides by Ehrlich cells.
MedLine Citation:
PMID:  4369426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Much of the lipid present in the ascites plasma in which Ehrlich cells grow is contained in very low density lipoproteins (VLDL). Chemical measurements indicated that triglycerides were taken up by the cells during in vitro incubation with ascites VLDL. When tracer amounts of radioactive triolein were incorporated into the ascites VLDL, the percentage uptakes of glyceryl tri[1-(14)C]oleate and triglycerides measured chemically were similar. The cells also took up [2-(3)H]glyceryl trioleate that was added to VLDL, but the percentage of available (3)H recovered in the cell lipids was 30-40% less than that of (1 4)C from glyceryl tri[1-(1 4)C]oleate. This difference was accounted for by water-soluble (3)H that accumulated in the incubation medium, suggesting that extensive hydrolysis accompanied the uptake of VLDL triglycerides. Radioactive fatty acids derived from the VLDL triglycerides were incorporated into cell phospholipids, glycerides, and free fatty acids, and they also were oxidized to CO(2). Triglyceride utilization increased as the VLDL concentration was raised. These results suggest that one function of the ascites plasma VLDL may be to supply fatty acid to the Ehrlich cells and that the availability of fatty acid to this tumor is determined in part by the ascites plasma VLDL concentration. Although Ehrlich cells incorporate almost no free glycerol into triglycerides, considerable amounts of [2-(3)H]glyceryl trioleate radioactivity were recovered in cell triglycerides. This indicates that at least some VLDL triglycerides were taken up intact. The net uptake of VLDL protein and cholesterol was very small relative to the triglyceride uptake, suggesting that intact triglycerides are transferred from the ascites VLDL to the Ehrlich cells and that hydrolysis occurs after the triglyceride is associated with the cells.
Authors:
D E Brenneman; A A Spector
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  15     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1974 Jul 
Date Detail:
Created Date:  1974-10-17     Completed Date:  1974-10-17     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  309-16     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / metabolism
Carbon Radioisotopes
Carcinoma, Ehrlich Tumor / metabolism*
Cells, Cultured
Cholesterol / metabolism
Culture Media
Electrophoresis
Fatty Acids, Nonesterified / metabolism
Lipoproteins, VLDL / isolation & purification,  metabolism*
Mice
Mice, Inbred CBA
Oxidation-Reduction
Phospholipids / metabolism
Time Factors
Triglycerides / metabolism*
Triolein / metabolism
Tritium
Ultracentrifugation
Valine / metabolism
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Culture Media; 0/Fatty Acids, Nonesterified; 0/Lipoproteins, VLDL; 0/Phospholipids; 0/Triglycerides; 10028-17-8/Tritium; 122-32-7/Triolein; 124-38-9/Carbon Dioxide; 57-88-5/Cholesterol; 7004-03-7/Valine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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