Document Detail


Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions.
MedLine Citation:
PMID:  18217938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To investigate amino acid and dipeptide utilization by Lactobacillus plantarum N4 isolated from orange peel, in a nutritionally depleted medium based on MRS (Mann, Rogosa, Sharpe). METHODS AND RESULTS: In MRS with 0.1 g l(-1) of meat extract and without peptone and yeast extract, growth increased when essential and stimulatory amino acids and nonessential amino acid were added to the medium. Replacement of the essential amino acid, leucine, and the nonessential amino acid, glycine, by leucyl-leucine (Leu-Leu) and/or glycyl-glycine (Gly-Gly) significantly enhanced growth. Essential amino acids were mainly consumed and the dipeptides were almost completely used at the end of growth. Leucine and glycine accumulated internally from the peptides were higher than from the free amino acids. Glucose utilization increased in the media containing dipeptides compared with the medium containing free amino acids. CONCLUSIONS: In a N-depleted medium, Leu-Leu and/or Gly-Gly were more effective than the respective amino acids in supporting growth of the micro-organism. The more efficient internal accumulation of glycine and especially leucine from dipeptides confirmed the ability of the strain to assimilate mainly complex nitrogen molecules rather than simple ones. SIGNIFICANCE AND IMPACT OF THE STUDY: The ability of Lact. plantarum N4 to efficiently use dipeptides could contribute to spoilage development in the natural medium of the organism, orange juice.
Authors:
F M Saguir; I E Loto Campos; M C Manca de Nadra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-23
Journal Detail:
Title:  Journal of applied microbiology     Volume:  104     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-22     Completed Date:  2008-10-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1597-604     Citation Subset:  IM    
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Bacteriological Techniques
Beverages*
Citrus*
Culture Media
Dipeptides / metabolism*
Fermentation
Food Microbiology*
Glucose / metabolism
Lactobacillus plantarum / growth & development*,  metabolism
Meat Products
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Dipeptides; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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