| Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions. | |
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MedLine Citation:
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PMID: 18217938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To investigate amino acid and dipeptide utilization by Lactobacillus plantarum N4 isolated from orange peel, in a nutritionally depleted medium based on MRS (Mann, Rogosa, Sharpe). METHODS AND RESULTS: In MRS with 0.1 g l(-1) of meat extract and without peptone and yeast extract, growth increased when essential and stimulatory amino acids and nonessential amino acid were added to the medium. Replacement of the essential amino acid, leucine, and the nonessential amino acid, glycine, by leucyl-leucine (Leu-Leu) and/or glycyl-glycine (Gly-Gly) significantly enhanced growth. Essential amino acids were mainly consumed and the dipeptides were almost completely used at the end of growth. Leucine and glycine accumulated internally from the peptides were higher than from the free amino acids. Glucose utilization increased in the media containing dipeptides compared with the medium containing free amino acids. CONCLUSIONS: In a N-depleted medium, Leu-Leu and/or Gly-Gly were more effective than the respective amino acids in supporting growth of the micro-organism. The more efficient internal accumulation of glycine and especially leucine from dipeptides confirmed the ability of the strain to assimilate mainly complex nitrogen molecules rather than simple ones. SIGNIFICANCE AND IMPACT OF THE STUDY: The ability of Lact. plantarum N4 to efficiently use dipeptides could contribute to spoilage development in the natural medium of the organism, orange juice. |
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Authors:
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F M Saguir; I E Loto Campos; M C Manca de Nadra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-23 |
Journal Detail:
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Title: Journal of applied microbiology Volume: 104 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-22 Completed Date: 2008-10-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1597-604 Citation Subset: IM |
Affiliation:
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Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Animals Bacteriological Techniques Beverages* Citrus* Culture Media Dipeptides / metabolism* Fermentation Food Microbiology* Glucose / metabolism Lactobacillus plantarum / growth & development*, metabolism Meat Products |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 0/Dipeptides; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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