| Utilization of amino acids by Trypanosoma brucei in culture: L-threonine as a precursor for acetate. | |
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MedLine Citation:
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PMID: 1187188 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The amino acid compositions of several culture media have been analysed and compared. The utilization and excretion of amino acids and other metabolites have been followed during growth of Trypanosoma brucei S42 in a defined medium. All of the added L-threonine was metabolized by the cells, even when it was present at elevated concentrations. Glucose was consumed throughout the growth cycle: glutamine was consumed more rapidly than glutamic acid, which was itself used at about the same rate as proline. Threonine was cleaved to form glycine and acetate, both of which accumulated in the medium. Alanine and succinate were excreted together with a small amount of pyruvate, but these three products accounted for less than half of the glucose used. CO2 production from glucose was not measured, but insignificant amounts of CO2 were produced from threonine. Tetraethylthiuram disulphide blocked the cleavage of threonine and was a potent inhibitor of trypanosome growth. |
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Authors:
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G A Cross; R A Klein; D J Linstead |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Parasitology Volume: 71 ISSN: 0031-1820 ISO Abbreviation: Parasitology Publication Date: 1975 Oct |
Date Detail:
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Created Date: 1976-01-14 Completed Date: 1976-01-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0401121 Medline TA: Parasitology Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 311-26 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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biosynthesis* Amino Acids / metabolism* Animals Carbon Dioxide / metabolism Culture Media Disulfiram / pharmacology Glutamates / metabolism Glutamine / metabolism Protein Biosynthesis Threonine / metabolism* Trypanosoma brucei brucei / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Amino Acids; 0/Culture Media; 0/Glutamates; 124-38-9/Carbon Dioxide; 56-85-9/Glutamine; 72-19-5/Threonine; 97-77-8/Disulfiram |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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