Document Detail


Utilization of DNA as a sole source of phosphorus, carbon, and energy by Shewanella spp.: ecological and physiological implications for dissimilatory metal reduction.
MedLine Citation:
PMID:  18156329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The solubility of orthophosphate (PO4(3-)) in iron-rich sediments can be exceedingly low, limiting the bioavailability of this essential nutrient to microbial populations that catalyze critical biogeochemical reactions. Here we demonstrate that dissolved extracellular DNA can serve as a sole source of phosphorus, as well as carbon and energy, for metal-reducing bacteria of the genus Shewanella. Shewanella oneidensis MR-1, Shewanella putrefaciens CN32, and Shewanella sp. strain W3-18-1 all grew with DNA but displayed different growth rates. W3-18-1 exhibited the highest growth rate with DNA. While strain W3-18-1 displayed Ca2+-independent DNA utilization, both CN32 and MR-1 required millimolar concentrations of Ca2+ for growth with DNA. For S. oneidensis MR-1, the utilization of DNA as a sole source of phosphorus is linked to the activities of extracellular phosphatase(s) and a Ca2+-dependent nuclease(s), which are regulated by phosphorus availability. Mass spectrometry analysis of the extracellular proteome of MR-1 identified one putative endonuclease (SO1844), a predicted UshA (bifunctional UDP-sugar hydrolase/5' nucleotidase), a predicted PhoX (calcium-activated alkaline phosphatase), and a predicted CpdB (bifunctional 2',3' cyclic nucleotide 2' phosphodiesterase/3' nucleotidase), all of which could play important roles in the extracellular degradation of DNA under phosphorus-limiting conditions. Overall, the results of this study suggest that the ability to use exogenous DNA as the sole source of phosphorus is widespread among the shewanellae, and perhaps among all prokaryotes, and may be especially important for nutrient cycling in metal-reducing environments.
Authors:
Grigoriy E Pinchuk; Christine Ammons; David E Culley; Shu-Mei W Li; Jeff S McLean; Margaret F Romine; Kenneth H Nealson; Jim K Fredrickson; Alexander S Beliaev
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-12-21
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-12     Completed Date:  2008-03-18     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1198-208     Citation Subset:  IM    
Affiliation:
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
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MeSH Terms
Descriptor/Qualifier:
Carbon / metabolism*
Chromatography, High Pressure Liquid
DNA / metabolism*
DNA Primers / genetics
Energy Metabolism / physiology*
Iron / metabolism
Mass Spectrometry
Oxidation-Reduction
Phosphorus / metabolism*
Polymerase Chain Reaction
Shewanella / enzymology,  growth & development*,  metabolism*
Species Specificity
Chemical
Reg. No./Substance:
0/DNA Primers; 7439-89-6/Iron; 7440-44-0/Carbon; 7723-14-0/Phosphorus; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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