| Utilization of 5-bromouracil by thymineless bacteria. | |
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MedLine Citation:
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PMID: 5335905 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an "absolute" thymine requirement could be easily distinguished from one with a "leaky" thymine requirement. |
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Authors:
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R Hewitt; J C Suit; D Billen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 93 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1967 Jan |
Date Detail:
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Created Date: 1967-05-10 Completed Date: 1967-05-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 86-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bromine
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metabolism Carbon Isotopes DNA, Bacterial / biosynthesis* Escherichia coli / metabolism* Mutation Thymine / metabolism* Tritium Ultracentrifugation Uracil / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/DNA, Bacterial; 10028-17-8/Tritium; 65-71-4/Thymine; 66-22-8/Uracil; 7726-95-6/Bromine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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