Document Detail


Utility of rodent models for evaluating protein allergenicity.
MedLine Citation:
PMID:  18957311     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Animal models are needed to assess novel proteins produced through biotechnology for potential dietary allergenicity. The exact characteristics that give certain foods allergenic potential are unclear, but must include both the potential to sensitize (induce IgE) as well as the capacity to avoid induction of oral tolerance (specific inhibition of IgE production). EPA has developed two complementary mouse models; one which distinguishes allergenic from non-allergenic food extracts using oral sensitization with adjuvant (cholera toxin) and another which further distinguishes highly potent allergens following oral administration without adjuvant based on the development (or not) of tolerance. For the foods tested thus far (roasted or raw peanut, Brazil nut, egg white, turkey, and spinach), the ability to sensitize and/or tolerize in these models are consistent with observed allergenicity as well as persistence and severity among allergens. Additionally, in vitro pepsin-resistance analysis of these food extracts suggests an association between sensitization capacity and proteins which are stable to gastric digestion. A subcutaneous exposure model did not distinguish allergenic from non-allergenic foods and does not appear useful for assessing potential allergenicity.
Authors:
Christal C Bowman; MaryJane K Selgrade
Publication Detail:
Type:  Journal Article; Review     Date:  2008-10-17
Journal Detail:
Title:  Regulatory toxicology and pharmacology : RTP     Volume:  54     ISSN:  1096-0295     ISO Abbreviation:  Regul. Toxicol. Pharmacol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8214983     Medline TA:  Regul Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S58-61     Citation Subset:  IM    
Affiliation:
Immunotoxicology Branch, Experimental Toxicology Division, US EPA/National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA. bowman.christal@epa.gov
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology*
Animals
Digestion
Female
Food Hypersensitivity / etiology*
Mice
Mice, Inbred C3H
Models, Animal*
Proteins / immunology*
Risk Assessment
Chemical
Reg. No./Substance:
0/Allergens; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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