| Utility of polymerase chain reaction in diagnosis of tuberculosis in our setup: a ten years experience. | |
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MedLine Citation:
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PMID: 22538034 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective: To evaluate the yield of polymerase chain reaction (PCR) for detection of Mycobacterium tuberculosis from different clinical specimens. Study Design: Observational study. Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi, from January 2001 to December 2010. Methodology: Different clinical specimens received for Mycobacterium tuberculosis PCR were dealt during the study period. Contaminated samples like sputum were processed by the standard N-acetyl L-cysteine (NALC)-NaOH method. PCR protocols were followed as per manufacturer's manual. PCR was performed using a Thermal Cycler (Master Cycler, Eppendorf, Germany): an initial denaturation step at 94°C for 3 minutes was followed by 40 cycles of denaturation at 94°C for 30 seconds, annealing at 60°C for 30 seconds and extension at 72°C for 30 seconds and a final extension at 72°C for 7 minutes. The products were held at 4°C and later run on 1% agarose gel, stained with Ethidium bromide and visualized in ultraviolet (UV) transilluminator. Results: Out of a total 4620 samples for PCR, 299 were positive for Mycobacterium tuberculosis (6.5%). The percentage of samples from male patients were 63.2%. The mean age of patients was 38+11.5 years. Blood was the most frequent specimen received for PCR (46.66%), followed by body fluids (18.41%) and CSF (10.64%). Yield for different clinical samples was 63/471 for sputum (13.4%), 3/29 for endobronchial washings (10.3%), 59/851 for body fluids (6.9%) and 24/400 for urine (6%). Positive yield from blood was the lowest (101/2156, 4.7%). Conclusion: PCR for Mycobacterium tuberculosis is a rapid and reliable method for the diagnosis of both pulmonary and extrapulmonary tuberculosis. The highest positive yield was obtained from sputum and lowest from blood specimens. |
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Authors:
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Faisal Hanif; Aamer Ikram; Luqman Satti; Muhammad Roshan; Shahid Ahmad Abbasi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP Volume: 22 ISSN: 1681-7168 ISO Abbreviation: J Coll Physicians Surg Pak Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-04-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9606447 Medline TA: J Coll Physicians Surg Pak Country: Pakistan |
Other Details:
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Languages: eng Pagination: 298-301 Citation Subset: IM |
Affiliation:
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Department of Pathology, Combined Military Hospital, Nowshera. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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