Document Detail

Utility of multiple sampling in reducing variation of salivary interleukin-8 and interleukin-1β mRNA levels in healthy adults.
MedLine Citation:
PMID:  22807130     Owner:  NLM     Status:  Publisher    
BACKGROUND: Salivary mRNA biomarkers can exhibit significant variation over time. The purpose of this study was to investigate the pattern and magnitude of variation of salivary mRNA biomarkers and to explore the effect of performing replicate assays and multiple sampling on the dispersion of salivary biomarkers. METHODS: Salivary interleukin-8 (IL-8) and interleukin-1β (IL-1β) mRNAs, developed as biomarkers for oral cancer detection, were selected and measured from 15 healthy subjects at 4 different time points on the same day. RESULTS: Salivary IL-8 and IL-1β mRNA levels fluctuated randomly over time without predictable rhythm and showed higher within-subject biological variation than analytical variation. The dispersion of salivary IL-8 and IL-1β mRNAs was more effectively reduced by taking multiple saliva sample measurements than by performing replicative assays. CONCLUSION: Taking multiple saliva samples can effectively reduce variation of salivary IL-8 and IL-1β mRNA levels in healthy adults. © 2012 Wiley Periodicals, Inc. Head Neck, 2012.
Jeong Kyu Kim; Hui Zhou; Vishad Nabili; Marilene B Wang; Elliot Abemayor; David T W Wong
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-17
Journal Detail:
Title:  Head & neck     Volume:  -     ISSN:  1097-0347     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8902541     Medline TA:  Head Neck     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Department of Otolaryngology - Head and Neck Surgery, Catholic University of Daegu School of Medicine, 3056-6 Daemyung 4-Dong, Nam-gu, Daegu, South Korea.
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