Document Detail

Utility of dual-modality bioluminescence and MRI in monitoring stem cell survival and impact on post myocardial infarct remodeling.
MedLine Citation:
PMID:  21145025     Owner:  NLM     Status:  MEDLINE    
RATIONALE AND OBJECTIVES: Firefly luciferase (Fluc) reporter gene is an authentic marker for surviving stem cells. However, it is unable to visualize the intramyocardial delivery of stem cells or their impact on cardiac function. The investigators demonstrate that bioluminescence imaging (BLI) combined with magnetic resonance imaging (MRI) allows better assessment of cell delivery and the impact on post-myocardial infarction remodeling.
MATERIALS AND METHODS: Murine embryonic stem cells (0.3 million) were double-labeled with Fluc and superparamagnetic iron oxide particles and injected into the infarct border zone of athymic rat hearts. BLI and MRI were performed serially up to 2 months after injection, followed by immunohistochemistry.
RESULTS: Dual-modality imaging was able to verify the initial intramyocardial delivery of the cells and their survival status. Over time, BLI signal increased in seven of nine hearts and disappeared in the other two hearts. The divergence of BLI signal over time was supported by MRI findings. Left ventricular ejection fraction and fractional shortening estimated by MRI suggested that cell engraftment mediated a positive impact on post-myocardial infarction remodeling. Two months after intramyocardial injection, superparamagnetic iron oxide-associated signals facilitated the localization of the injection site.
CONCLUSIONS: Dual-modality imaging has the unique ability to monitor cell delivery, survival status, graft morphology, and impact on post-myocardial infarction remodeling.
Hualei Zhang; Hui Qiao; Ashley Bakken; Fabao Gao; Bin Huang; Yingqiu Y Liu; Wafik El-Deiry; Victor A Ferrari; Rong Zhou
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Academic radiology     Volume:  18     ISSN:  1878-4046     ISO Abbreviation:  Acad Radiol     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  2011-04-19     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9440159     Medline TA:  Acad Radiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3-12     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 AUR. Published by Elsevier Inc. All rights reserved.
Department of Radiology, University of Pennsylvania, Philadelphia, 19104, USA.
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MeSH Terms
Cell Survival*
Contrast Media / diagnostic use
Dextrans / diagnostic use
Disease Models, Animal
Genes, Reporter
Luciferases, Firefly / diagnostic use
Luminescent Agents / diagnostic use
Luminescent Measurements / methods*
Magnetic Resonance Imaging / methods*
Magnetite Nanoparticles / diagnostic use
Myocardial Infarction / physiopathology*
Rats, Nude
Stem Cells / cytology*
Ventricular Remodeling*
Grant Support
R01 HL081185-01A1/HL/NHLBI NIH HHS; R01 HL081185-02/HL/NHLBI NIH HHS; R01 HL081185-03/HL/NHLBI NIH HHS; R01 HL081185-04/HL/NHLBI NIH HHS; R01 HL081185-04S1/HL/NHLBI NIH HHS; R01-HL081185/HL/NHLBI NIH HHS; R21 EB002473-01/EB/NIBIB NIH HHS; R21 EB002473-02/EB/NIBIB NIH HHS; R21EB-2473/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Contrast Media; 0/Luminescent Agents; 0/Magnetite Nanoparticles; 9004-54-0/Dextrans; EC, Firefly; G6N3J05W84/ferumoxides
Comment In:
Acad Radiol. 2011 Jan;18(1):1-2   [PMID:  21145024 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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