| Utility of adenovirus-mediated Fas ligand and bcl-2 gene transfer to modulate rat liver allograft survival. | |
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MedLine Citation:
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PMID: 17085333 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Expression of Fas ligand (FasL) on the graft by gene transduction is expected to introduce apoptosis to lymphocytes to protect rejection, but the FasL-expressing graft cells may also induce apoptosis as the graft usually expresses Fas antigens. In this study, a strong antiapoptotic gene, bcl-2, was cotransfected with the FasL gene in rat liver graft to protect against Fas-mediated cell death and to prolong recipient survival. METHODS: Orthotopic liver transplantation was done in a strain combination of DA to LEW rats. After donor vascular isolation, adenovirus-mediated FasL and bcl-2 genes were cotransfected in the liver graft. RESULTS: Intragraft expression of FasL mRNA was constitutively expressed after adenovirus-mediated transduction, although expression of FasL increased mildly in control grafts. Bcl-2 mRNA was highly expressed at 2 days after reperfusion. In contrast, lower expression of bcl-2 was observed in the control group. The average survival of the gene transferred allografts increased from (9.8+1.3) days to (18.5+8.7) days compared with the control group. CONCLUSION: Our results indicate that rat liver allografts can be protected against host immune responses by adenovirus-mediated FasL and bcl-2 transfection, and that bcl-2 expression prevents the graft from Fas-mediated apoptosis. |
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Authors:
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De-Sheng Wang; Yu Li; Ke-Feng Dou; Kai-Zong Li; Zhen-Shun Song |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hepatobiliary & pancreatic diseases international : HBPD INT Volume: 5 ISSN: 1499-3872 ISO Abbreviation: HBPD INT Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-06 Completed Date: 2007-02-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101151457 Medline TA: Hepatobiliary Pancreat Dis Int Country: China |
Other Details:
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Languages: eng Pagination: 505-10 Citation Subset: IM |
Affiliation:
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Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. wangdesh@163.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Animals Apoptosis / genetics Fas Ligand Protein / genetics* Genes, bcl-2* Graft Rejection / pathology Liver / pathology Liver Transplantation / immunology* Male Rats Rats, Inbred Lew Reverse Transcriptase Polymerase Chain Reaction Transfection* Transplantation Tolerance* Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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0/Fas Ligand Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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