| Utilisation of fatty acid and triacylglycerol by rat macrophages: the effect of endotoxin. | |
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MedLine Citation:
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PMID: 12438765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: Plasma very-low-density lipoprotein (VLDL) concentrations are increased during sepsis/endotoxaemia and VLDL may be substrates for the activated immune system. Lipid substrate utilisation by quiescent and activated macrophages was therefore examined. METHODS: Rat R2 macrophages were incubated with oleate or rat VLDL, with or without lipopolysaccharide (LPS) stimulation. The metabolic fate of the lipids was examined. RESULTS: Macrophages utilised both oleate and (control) VLDL-triacylglycerol (TAG) to a similar extent. Most was deposited as cellular lipid; about 10% of oleate was oxidised compared to 25% of cVLDL-TAG. LPS significantly increased oleate oxidation and its deposition as cellular lipid (mostly as TAG) at 48hrs but abolished both oxidation and storage of VLDL-TAG. VLDL produced during endotoxaemia (eVLDL) was assimilated by the unstimulated macrophage to a greater extent than oleate or cVLDL-TAG and selectively stored as cellular lipids (no oxidation); LPS decreased eVLDL utilisation. VLDL-TAG utilisation was decreased by the lipoprotein lipase (LPL) inhibitor terahydrolipstatin. LPL activity was greater in oleate than in VLDL incubations, but was increased by incubation with eVLDL. LPS had no effect (oleate, cVLDL) or increased (eVLDL) LPL activity. CONCLUSION: VLDL represented an efficient substrate for the macrophage. However under conditions of sepsis/endotoxaemia eVLDL utilisation was limited and directed away from oxidation, suggesting that the increased plasma TAG (eVLDL) concentrations resulting from sepsis is not a respiratory fuel for the macrophage but may supply cellular lipid. |
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Authors:
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David Hauton; Rhys D Evans |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 12 ISSN: 1015-8987 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-11-19 Completed Date: 2003-06-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
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Languages: eng Pagination: 293-304 Citation Subset: IM |
Copyright Information:
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Copyright 2002 S. Karger AG, Basel |
Affiliation:
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Nuffield Department of Anaesthetics, University of Oxford, Radcliffe Infirmary, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Endotoxins / biosynthesis, pharmacology* Fatty Acids / metabolism* Glucose / metabolism Lactones / pharmacology Lipopolysaccharides / pharmacology Lipoprotein Lipase / antagonists & inhibitors, metabolism Lipoproteins, VLDL / metabolism* Liver / metabolism Macrophages / drug effects, enzymology, metabolism* Male Oleic Acid / metabolism Oxidation-Reduction Rats Rats, Wistar Triglycerides / metabolism* Triolein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Endotoxins; 0/Fatty Acids; 0/Lactones; 0/Lipopolysaccharides; 0/Lipoproteins, VLDL; 0/Triglycerides; 112-80-1/Oleic Acid; 122-32-7/Triolein; 50-99-7/Glucose; 96829-58-2/orlistat; EC 3.1.1.34/Lipoprotein Lipase |
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