Document Detail


Utilisation of fatty acid and triacylglycerol by rat macrophages: the effect of endotoxin.
MedLine Citation:
PMID:  12438765     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Plasma very-low-density lipoprotein (VLDL) concentrations are increased during sepsis/endotoxaemia and VLDL may be substrates for the activated immune system. Lipid substrate utilisation by quiescent and activated macrophages was therefore examined. METHODS: Rat R2 macrophages were incubated with oleate or rat VLDL, with or without lipopolysaccharide (LPS) stimulation. The metabolic fate of the lipids was examined. RESULTS: Macrophages utilised both oleate and (control) VLDL-triacylglycerol (TAG) to a similar extent. Most was deposited as cellular lipid; about 10% of oleate was oxidised compared to 25% of cVLDL-TAG. LPS significantly increased oleate oxidation and its deposition as cellular lipid (mostly as TAG) at 48hrs but abolished both oxidation and storage of VLDL-TAG. VLDL produced during endotoxaemia (eVLDL) was assimilated by the unstimulated macrophage to a greater extent than oleate or cVLDL-TAG and selectively stored as cellular lipids (no oxidation); LPS decreased eVLDL utilisation. VLDL-TAG utilisation was decreased by the lipoprotein lipase (LPL) inhibitor terahydrolipstatin. LPL activity was greater in oleate than in VLDL incubations, but was increased by incubation with eVLDL. LPS had no effect (oleate, cVLDL) or increased (eVLDL) LPL activity. CONCLUSION: VLDL represented an efficient substrate for the macrophage. However under conditions of sepsis/endotoxaemia eVLDL utilisation was limited and directed away from oxidation, suggesting that the increased plasma TAG (eVLDL) concentrations resulting from sepsis is not a respiratory fuel for the macrophage but may supply cellular lipid.
Authors:
David Hauton; Rhys D Evans
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  12     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2002  
Date Detail:
Created Date:  2002-11-19     Completed Date:  2003-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  293-304     Citation Subset:  IM    
Copyright Information:
Copyright 2002 S. Karger AG, Basel
Affiliation:
Nuffield Department of Anaesthetics, University of Oxford, Radcliffe Infirmary, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Endotoxins / biosynthesis,  pharmacology*
Fatty Acids / metabolism*
Glucose / metabolism
Lactones / pharmacology
Lipopolysaccharides / pharmacology
Lipoprotein Lipase / antagonists & inhibitors,  metabolism
Lipoproteins, VLDL / metabolism*
Liver / metabolism
Macrophages / drug effects,  enzymology,  metabolism*
Male
Oleic Acid / metabolism
Oxidation-Reduction
Rats
Rats, Wistar
Triglycerides / metabolism*
Triolein / metabolism
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Fatty Acids; 0/Lactones; 0/Lipopolysaccharides; 0/Lipoproteins, VLDL; 0/Triglycerides; 112-80-1/Oleic Acid; 122-32-7/Triolein; 50-99-7/Glucose; 96829-58-2/orlistat; EC 3.1.1.34/Lipoprotein Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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