Document Detail


Uteroglobin production by cultured rabbit uterine epithelial cells.
MedLine Citation:
PMID:  6832058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Uteroglobin (UG) is a secretory protein produced by the rabbit endometrium and its production is increased during cell differentiation which occurs during early pregnancy or pseudopregnancy. In the present study, the optimal conditions for UG production by rabbit endometrial epithelial cells in culture were examined. Metabolic labeling studies showed the incorporation of [35S]methionine into UG molecules by the endometrial epithelial cells in culture. Accumulation of UG in culture media was linear for at least a period of 24 h. These cells do not catabolize exogenously added radiolabeled UG. Endometrial cells obtained from virgin female rabbits at different times after the administration of human CG (hCG) and put in culture were found to make different amounts of UG. The maximal UG production was found in cells taken from pseudopregnant rabbits 4 days after hCG administration. Cycloheximide (28 micrograms/ml) inhibited the production of UG by the cells in culture whereas actinomycin-D (5 micrograms/ml) and cordycipin (50 micrograms/ml) increased its production. Inhibition of DNA synthesis by hydroxyurea (10(-3) M) did not affect the UG production. The production of UG was significantly less when cells were cultured on attached or floating collagen gels as compared to cells grown on plastic Petri dishes. The amino acid content of Ham's F-12 medium was shown to be adequate for maximal UG production; lowering this amino acid concentration decreased the amount of UG accumulated in the medium over a 24-h period. Increasint the number of cultured cells per dish resulted in an increased UG production per cell.
Authors:
K Rajkumar; R Bigsby; R Lieberman; L E Gerschenson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  112     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1490-8     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chorionic Gonadotropin / pharmacology
Collagen
Culture Media
Epithelium / metabolism
Female
Glycoproteins / biosynthesis*
Ovulation / drug effects
Rabbits
Uteroglobin / biosynthesis*
Uterus / metabolism*
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Culture Media; 0/Glycoproteins; 9007-34-5/Collagen; 9060-09-7/Uteroglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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