Document Detail


Uterine and hepatic gene expression in relation to days postpartum, estrus, and pregnancy in postpartum dairy cows.
MedLine Citation:
PMID:  18096935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The somatotropic axis consisting of growth hormone, the growth hormone receptor (GHR) insulin-like growth factor (IGF)-I, and IGF binding proteins changes with the stage of lactation and nutrition of the cow and may be 1 mechanism through which lactation and nutrition affect the establishment of pregnancy. The objective of this study was to quantify GHR, IGF-I, and IGF binding protein-2 (IGFBP-2) mRNA in liver and uterine endometrial tissue at 4 stages of lactation (40, 80, 120, and 160 days in milk) and around the time of artificial insemination. Estrus was synchronized with GnRH and PGF2alpha, and cows were inseminated 12 h after estrus. Uterine biopsies were collected immediately before the second injection of PGF2alpha (before estrus), at the initiation of standing estrus, and 4 d after estrus. Liver biopsies were collected once on 4 d after estrus. The abundance of GHR, IGF-I, and IGFBP-2 mRNA in liver and uterus was determined by real-time quantitative PCR. The amount of liver IGF-I mRNA was positively correlated with plasma IGF-I concentrations. Cows that became pregnant after AI had more GHR and IGFBP-2 mRNA in their liver than cows that did not become pregnant. There was no effect of DIM or pregnancy status on abundance of uterine mRNA; however, uterine GHR and IGF-I mRNA was most abundant at estrus. In summary, cows at different stages of lactation or with different pregnancy statuses had similar quantities of uterine mRNA. In contrast, liver quantities of mRNA differed relative to pregnancy status. These data provide evidence that liver indices of metabolic state may be indicative of pregnancy success.
Authors:
M L Rhoads; J P Meyer; W R Lamberson; D H Keisler; M C Lucy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of dairy science     Volume:  91     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-21     Completed Date:  2008-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  140-50     Citation Subset:  IM    
Affiliation:
Division of Animal Sciences, University of Missouri, Columbia 65211, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopsy / veterinary
Cattle / physiology*
Endometrium / metabolism,  physiology*
Estrous Cycle / genetics*,  metabolism
Female
Gene Expression Regulation / physiology*
Insulin-Like Growth Factor Binding Protein 2 / biosynthesis*,  genetics
Insulin-Like Growth Factor I / biosynthesis*,  genetics
Lactation / physiology
Liver / metabolism,  physiology*
Pregnancy
RNA, Messenger / biosynthesis,  genetics
Receptors, Somatotropin / biosynthesis*,  genetics
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Chemical
Reg. No./Substance:
0/Insulin-Like Growth Factor Binding Protein 2; 0/RNA, Messenger; 0/Receptors, Somatotropin; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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