Document Detail


Uterine epithelial morphology and progesterone receptors in a mifepristone-treated viviparous lizard Pseudemoia entrecasteauxii (Squamata: Scincidae) during gestation.
MedLine Citation:
PMID:  22532476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Structural and functional changes to the uterus associated with maintenance of pregnancy are controlled primarily by steroid hormones such as progesterone. We tested the hypothesis that progesterone regulates uterine structural changes during pregnancy in the viviparous skink, Pseudemoia entrecasteauxii, by treating pregnant females with the progesterone receptor antagonist mifepristone at different stages of pregnancy. Expression and distribution of progesterone receptor was determined using Western blot and immunohistochemistry. During early pregnancy, mifepristone treatment resulted in altered uterine epithelial cell surface morphology and high embryo mortality, but did not affect females at mid and late stages of pregnancy. Females treated with mifepristone in early pregnancy exhibited abnormal uterine epithelial cell morphology such as lateral blebbing and presence of wide gaps between cells indicating loss of intercellular attachment. Chorioallantoic membranes of the embryo were not affected by mifepristone treatment. Two isoforms (55 kDa and 100 kDa) of progesterone receptor were identified using immunoblots and both isoforms were localized to the nucleus of uterine epithelial cells. The 55 kDa isoform was expressed throughout pregnancy, whereas the 100 kDa isoform was expressed during mid and especially late pregnancy. In P. entrecasteauxii, mifepristone may prevent successful embryo attachment in early pregnancy through its effects on uterine epithelial cells but may have little effect on pregnancy once the maternal-embryo structural relationship is established.
Authors:
Joanna M Biazik; Scott L Parker; Christopher R Murphy; Michael B Thompson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of experimental zoology. Part B, Molecular and developmental evolution     Volume:  318     ISSN:  1552-5015     ISO Abbreviation:  J. Exp. Zool. B Mol. Dev. Evol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-04-25     Completed Date:  2012-08-16     Revised Date:  2012-10-04    
Medline Journal Info:
Nlm Unique ID:  101168228     Medline TA:  J Exp Zool B Mol Dev Evol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  148-58     Citation Subset:  IM    
Copyright Information:
© 2011 WILEY PERIODICALS, INC.
Affiliation:
School of Biological Sciences, University of Sydney, Sydney, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Embryo, Nonmammalian / anatomy & histology,  drug effects*,  metabolism
Female
Hormone Antagonists / pharmacology*
Immunohistochemistry
Lizards* / anatomy & histology,  physiology
Microscopy, Electron, Scanning
Mifepristone / pharmacology*
Receptors, Progesterone / metabolism*
Uterus / anatomy & histology,  drug effects*,  metabolism
Viviparity, Nonmammalian* / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Hormone Antagonists; 0/Receptors, Progesterone; 84371-65-3/Mifepristone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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