Document Detail


Using zebrafish to learn statistical analysis and mendelian genetics.
MedLine Citation:
PMID:  21682600     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract This project was developed to promote understanding of how mathematics and statistical analysis are used as tools in genetic research. It gives students the opportunity to carry out hypothesis-driven experiments in the classroom: students generate hypotheses about Mendelian and non-Mendelian inheritance patterns, gather raw data, and test their hypotheses using chi-square statistical analysis. In the first protocol, students are challenged to analyze inheritance patterns using GloFish, brightly colored, commercially available, transgenic zebrafish that express Green, Yellow, or Red Fluorescent Protein throughout their muscles. In the second protocol, students learn about genetic screens, microscopy, and developmental biology by analyzing the inheritance patterns of mutations that cause developmental defects. The difficulty of the experiments can be adapted for middle school to upper level undergraduate students. Since the GloFish experiments use only fish and materials that can be purchased from pet stores, they should be accessible to many schools. For each protocol, we provide detailed instructions, ideas for how the experiments fit into an undergraduate curriculum, raw data, and example analyses. Our plan is to have these protocols form the basis of a growing and adaptable educational tool available on the Zebrafish in the Classroom Web site.
Authors:
Samantha Lindemann; Jon Senkler; Elizabeth Auchter; Jennifer O Liang
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zebrafish     Volume:  8     ISSN:  1557-8542     ISO Abbreviation:  Zebrafish     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101225070     Medline TA:  Zebrafish     Country:  United States    
Other Details:
Languages:  eng     Pagination:  41-55     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Minnesota Duluth , Duluth, Minnesota.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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