Document Detail

Using site-directed mutagenesis to probe the role of the D2 carotenoid in the secondary electron-transfer pathway of photosystem II.
Jump to Full Text
MedLine Citation:
PMID:  23334888     Owner:  NLM     Status:  Publisher    
Secondary electron transfer in photosystem II (PSII), which occurs when water oxidation is inhibited, involves redox-active carotenoids (Car), as well as chlorophylls (Chl), and cytochrome b (559) (Cyt b (559)), and is believed to play a role in photoprotection. Car(D2) may be the initial point of secondary electron transfer because it is the closest cofactor to both P(680), the initial oxidant, and to Cyt b (559), the terminal secondary electron donor within PSII. In order to characterize the role of Car(D2) and to determine the effects of perturbing Car(D2) on both the electron-transfer events and on the identity of the redox-active cofactors, it is necessary to vary the properties of Car(D2) selectively without affecting the ten other Car per PSII. To this end, site-directed mutations around the binding pocket of Car(D2) (D2-G47W, D2-G47F, and D2-T50F) have been generated in Synechocystis sp. PCC 6803. Characterization by near-IR and EPR spectroscopy provides the first experimental evidence that Car(D2) is one of the redox-active carotenoids in PSII. There is a specific perturbation of the Car(∙+) near-IR spectrum in all three mutated PSII samples, allowing the assignment of the spectral signature of Car (D2) (∙+) ; Car (D2) (∙+) exhibits a near-IR peak at 980 nm and is the predominant secondary donor oxidized in a charge separation at low temperature in ferricyanide-treated wild-type PSII. The yield of secondary donor radicals is substantially decreased in PSII complexes isolated from each mutant. In addition, the kinetics of radical formation are altered in the mutated PSII samples. These results are consistent with oxidation of Car(D2) being the initial step in secondary electron transfer. Furthermore, normal light levels during mutant cell growth perturb the shape of the Chl(∙+) near-IR absorption peak and generate a dark-stable radical observable in the EPR spectra, indicating a higher susceptibility to photodamage further linking the secondary electron-transfer pathway to photoprotection.
Katherine E Shinopoulos; Jianfeng Yu; Peter J Nixon; Gary W Brudvig
Related Documents :
18960938 - Identification of some polyatomic inorganic anions as their diphenyliodonium salts by i...
17352038 - Photoisomerization and thermal isomerization of arylazoimidazoles.
23730708 - Naphthoxanthenyl, a new stable phenalenyl type radical stabilized by electronic effects.
23339278 - The different nature of the interactions between anions and hat(cn)6: from reversible a...
12443108 - Scaling of the interface roughness in fe-cr superlattices: self-affine versus non-self-...
18247988 - Enhanced adsorption energy of au1 and o2 on the stoichiometric tio2(110) surface by coa...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  Photosynthesis research     Volume:  -     ISSN:  1573-5079     ISO Abbreviation:  Photosyn. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954728     Medline TA:  Photosynth Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Chemistry, Yale University, New Haven, CT, 06520-8107, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Photosynth Res
Journal ID (iso-abbrev): Photosyn. Res
ISSN: 0166-8595
ISSN: 1573-5079
Publisher: Springer Netherlands, Dordrecht
Article Information
Download PDF
© The Author(s) 2013
Received Day: 18 Month: 10 Year: 2012
Accepted Day: 2 Month: 1 Year: 2013
Electronic publication date: Day: 21 Month: 1 Year: 2013
pmc-release publication date: Day: 21 Month: 1 Year: 2013
Print publication date: Year: 2014
Volume: 120First Page: 141 Last Page: 152
PubMed Id: 23334888
ID: 3961632
Publisher Id: 9793
DOI: 10.1007/s11120-013-9793-6

Using site-directed mutagenesis to probe the role of the D2 carotenoid in the secondary electron-transfer pathway of photosystem II
Katherine E. ShinopoulosAff1
Jianfeng YuAff2
Peter J. NixonAff2
Gary W. BrudvigAff1 Address: +203-432-5202 +203-432-6144
Department of Chemistry, Yale University, New Haven, CT 06520-8107 USA
Division of Molecular Biosciences, Sir Ernst Chain Building – Wolfson Laboratories, Imperial College London, S. Kensington campus, London, SW7 2AY UK


Photosystem II (PSII) is the enzyme responsible for photosyntheic oxidation of water to O2, generating the reducing equivalents that ultimately are used for CO2 fixation. Water oxidation occurs when excitons are transferred to a special group of chlorophylls, known as P680, where a charge separation occurs, as seen in Fig. 1. The electron is transferred to PheoA on a timescale of tens of picoseconds (Holzwarth et al. 2006), and then to QA with a timescale of 200–500 picoseconds (ps) (Rappaport and Diner 2008). The electron–hole pair on P680+ and QA is stable for close to 1 ms in cyanobacteria (Reinman et al. 1981; Gerken et al. 1989; Metz et al. 1989), during which time, under catalytic conditions, the oxygen-evolving complex (OEC) donates an electron to P680+ via a redox-active tyrosine, YZ. Once the OEC, which consists of a Mn4CaO5 cluster (Umena et al. 2011), has been oxidized four times via sequential charge separations to reach a high-valent state, probably Mn(IV)Mn(IV)Mn(IV)Mn(IV)-O (Siegbahn 2006; Sproviero et al. 2008), it is capable of oxidizing water to dioxygen. Meanwhile, the electron on QA is transferred to QB, which dissociates away from PSII after two reductions and subsequent protonations, carrying reducing equivalents to the next step in photosynthesis and ultimately resulting in the storage of energy in the chemical bonds of sugars.

However, the intermediates associated with water splitting are very oxidizing, and cause damage to the protein over time. The D1 subunit of PSII, which contains most of the cofactors involved in water oxidation, turns over every 30 min, in a process that involves disassembly of the PSII complex, membrane diffusion, and protein synthesis (Nixon et al. 2010). In order to minimize damage, PSII has evolved multiple mechanisms of photoprotection to prolong the lifetime of its subunits and minimize energy expenditure for protein synthesis. One mechanism involves adjusting the size of the light-harvesting antenna; other mechanisms involve dissipating excess solar energy as heat, as in the xanthophyll cycle in plants (Niyogi 1999) or via the orange carotenoid protein in cyanobacteria (Kirilovsky and Kerfeld 2012). In addition, when water-oxidation catalysis is impaired, oxidation of secondary donors, including carotenoids (Car), chlorophylls (Chl), and cytochrome b559 (Cyt b559), may serve to remove excess oxidizing equivalents from PSII (Thompson and Brudvig 1988; Buser et al. 1992) or to quench chlorophyll excited states (Schweitzer and Brudvig 1997).

Of the secondary electron donors, Cyt b559 has the lowest reduction potential, which is interestingly variable from 390 mV to as low as −150 mV (Thompson et al. 1989; Stewart and Brudvig 1998). Cyt b559 is, therefore, the terminal secondary electron donor within PSII. It may additionally be rereduced by the plastoquinone pool, leading to a cyclic process for the removal of excess, damaging oxidizing equivalents from PSII when the system is unable to drive water oxidation (Shinopoulos and Brudvig 2012).

Although the final location of the oxidizing equivalent passed along the secondary electron-transfer pathway has been determined to be Cyt b559 (Vermeglio and Mathis 1974; de Paula et al. 1985), the pathway of electron transfer from Cyt b559 to P680+ has not been fully characterized. The distance of about 40 Å between the two cofactors indicates that they do not participate in direct electron transfer, and it has indeed been observed that Chl and Car are intermediates (de Paula et al. 1985; Hanley et al. 1999; Vrettos et al. 1999; Tracewell et al. 2001; Faller et al. 2001). It has also been shown that there are at least two redox-active carotenoids (Car∙+) in PSII based on the shift of the Car+ near-IR peak over a range of illumination temperatures and the wavelength-dependant decay rate of the Car+ absorbance (Tracewell and Brudvig 2003; Telfer et al. 2003). There are as many as 5 redox-active Chl (Chl∙+) (Tracewell and Brudvig 2008; Telfer et al. 1990), with one ligated to D1-His 118 (Stewart et al. 1998). However, there are 11 Car and 35 Chl per PSII, as seen in Fig. 2, and most of the redox-active cofactors have not been specifically identified. Some Chl∙+ may be in CP43 and CP47, peripheral subunits that bind many Chl molecules (Tracewell and Brudvig 2008). In regard to the two Car∙+, it has been observed that the average distance from the nonheme iron to the two Car∙+ is 38 Å, and it has been hypothesized that one Car∙+ is CarD2∙+ (Lakshmi et al. 2003; Tracewell and Brudvig 2003). This seems likely, because CarD2 is the closest cofactor to both P680 and Cyt b559, with edge-to-edge distances of 11 and 12 Å, respectively. The oxidation of YD results in a shift of the Car∙+ near-IR peak, indicating proximity of at least one Car∙+ to YD (Tracewell and Brudvig 2003), although electrochromic effects can propagate significant distances though PSII (Stewart et al. 2000). A relatively higher yield of Car∙+ than Chl∙+ is observed at lower temperatures, with increased Chl∙+ at higher temperatures, also indicating that Car∙+ is closer than Chl∙+ to P680 (Hanley et al. 1999).

In order to evaluate the role of CarD2 in secondary electron transfer relative to the roles of other Car in PSII, we have characterized the effects of site-directed mutations around the binding pocket of CarD2 (see Fig. 3). In this study, the effects of the mutations D2-G47W, D2-T50F, and D2-G47F on the secondary electron-transfer pathway are examined by low temperature near-IR optical and EPR spectroscopy.

Materials and methods
Chemicals and reagents

2-(N-morpholino)-ethanesulfonic acid (MES) was purchased from USB Corporation. β–Dodecyl maltoside (β-DM) was purchased from Enzo Life Sciences International Inc. A stock solution (80 mM) of potassium ferricyanide (purchased from Sigma-Aldrich) was prepared in buffer and frozen until use.


D2 mutants were constructed according to (Tang et al. 1993) except that the recipient strain Tol145/CP47-His, obtained by transforming strain Tol145 (Tang et al. 1993) with genomic DNA from strain PSII-His (Boehm et al. 2011), also encoded a C-terminal His-tagged derivative of CP47. Plasmid pDC074 was used as the parental vector for site-directed mutagenesis (Tang et al. 1993). Mutations were introduced into the plasmid by overlap-extension PCR so that the codon specifying D2-G47 was replaced by either TGG (to make mutated D2-G47W) or TTC (D2-G47F) and the codon specifying D2-T50 was replaced by TTC (D2-T50F). In all three cases, the codon for Leu45 (CTG) was mutated to incorporate a silent mutation (CTA), in order to create a unique restriction site, AvrII, to help screen for mutations. The genotypes of the cyanobacterial mutants were confirmed by PCR analysis and DNA sequencing.

Cell growth and protein purification

Cells were grown initially on plates containing 5 mM glucose, 10 μM DCMU, 25 mg/L kanamycin, and 10 mg/L erythromycin. In liquid culture, the cells were grown without antibiotics in the presence of 5 mM glucose under 10 or 40 μEinsteins/m2/s of illumination, as noted. His-tagged PSII core particles were isolated from Synechocystis PCC 6803 cells as previously described (Lakshmi et al. 2002).

Sample treatments

For low-temperature measurements, PSII samples were transferred to a buffer containing 15 mM CaCl2, 63 % (v/v) glycerol, and 50 mM MES at pH 6.0. Prior to freezing, PSII samples were treated with 5 mM ferricyanide to oxidize Cyt b559.

Near-IR optical spectroscopy

A Perkin-Elmer Lambda 20 spectrometer was used to make optical spectroscopic measurements in the visible and near-IR. Low-temperature optical measurements were made with an Oxford Instruments Optistat liquid helium cryostat. Polyethylene cuvettes with a 1.0 cm path length and 0.4 cm width (Fisher Scientific) were used for low-temperature optical measurements. A 150 W quartz-halogen lamp filtered by a 6 in water filter and a heat-absorbing filter (Schott KG-5) was used to illuminate samples. A Schott-Fostec randomized fiber optic bundle was used to direct the light into the cryostat. The PSII samples were prepared as previously described (Tracewell and Brudvig 2008). Illumination for 15 min was performed on samples that were equilibrated at the specified temperature for at least 60 min in the cryostat. All spectra collected after illumination are referenced to the dark spectrum measured at the same temperature to avoid contributions from spectral changes in the background due to temperature effects.

Spectral simulations

The program Igor Pro 6.2 was used to simulate the near-IR absorption data, to analyze the decay kinetics, and to plot all spectra.

EPR spectroscopy

X-band EPR measurements were conducted on a Bruker ELEXSYS E500 EPR spectrometer equipped with an Oxford ESR 900 He-flow cryostat and a Super High Q cavity. Samples were illuminated by a xenon halogen lamp filtered by a 6 in water filter and a heat-absorbing filter, with a fiber optic cable directing light into the cryostat. Radical yields per PSII were determined by integration of the derivative EPR signals and calibrated to photooxidized tyrosine D (YD). YD was generated by illuminating the PSII samples for 30 s at 0 °C, incubating on ice for 2 min, and freezing in total darkness.

Selection of mutations

The mutations D2-G47F, D2-G47W, and D2-T50F were selected by using Coot, a modeling program that includes the ability to mutate a selected residue from a known crystal structure (Emsley and Cowtan 2004). The mutated residue is placed in the conformation in which it is typically found, and other conformations are also observable. Using the 3.0-Å resolution crystal structure of PSII (Loll et al. 2005), which was focused on accurate cofactor positioning and found similar locations to the recent 1.9-Å resolution structure (Umena et al. 2011), residues that would sterically interfere with CarD2 binding were identified, as shown in Fig. 3. Aromatic residues have been observed around the β-ionylidene ring binding site (Tracewell and Brudvig 2003), which has been found to be important for function (Bautista et al. 2005), and because the Car chain exists in a variety of conformations in PSII samples, the area near the rings was targeted for mutation. In this way, several mutations were identified that may cause a disruption to the hydrophobic binding pocket of the β-ionylidene ring of CarD2.

Near-IR Optical Spectroscopy

WT, D2-T50F, D2-G47W, and D2-G47F His-tagged PSII complexes were illuminated in a cryostat at 20 K for 15 min, maximally generating one stable charge separation per PSII center; at this temperature in ferricyanide-treated samples, the stable charge separation results in an electron on QA and a hole that is located on either a Car neutral radical (Car absorbing at 750 nm), a Chl cation radical (Chl∙+ absorbing at 800–840 nm) or a Car cation radical (Car∙+ absorbing near 1,000 nm), as seen in Fig. 4. For each mutated PSII sample, the total yield of stable charge separated states was lower than in WT PSII samples when normalized to the same concentration of Chl, indicated by the lower yield of all secondary donors (Car, Chl∙+, and Car∙+), seen in Fig. 4A. When the magnitudes of the Car∙+ peaks are normalized to 1, as in Fig. 4B, it can be seen that the Car∙+ peak is slightly red shifted and has a larger FWHM in mutated PSII samples compared to WT PSII samples. The yield of the Car peak at 750 nm tracks with the magnitude of the Car∙+ peak, reinforcing that it is generated from Car (Gao et al. 2009). In the mutated PSII samples, there is slightly more Chl∙+ generated relative to Car∙+ than in WT, especially in the G47F and G47W PSII samples, with an absorbance centered at 825 nm. Although the yield of Chl∙+ appears to be very low, it has an extinction coefficient of about 7,000 M−1 cm−1 (Borg et al. 1970), while Car∙+ has an extinction coefficient of about 160,000 M−1 cm−1 (Tan et al. 1997). The width and shape of the Chl∙+ peak varies among the samples, as seen in Fig. 4C. The T50F PSII sample isolated from cells grown at 10 μEinsteins/m2/s of illumination has the narrowest peak, followed closely by G47F PSII samples. PSII samples isolated from G47W, T50F grown under 40 μEinsteins/m2/s of illumination, and WT cells display wider Chl∙+ signatures that appear to contain two peaks.

Using global analysis in Igor Pro 6.2, the Car∙+ peak in all PSII samples was deconvoluted into two Gaussian contributions. One contribution had a maximum at 999–1,003 nm, while the other varied from 980 nm in WT PSII to 993 nm in G47W PSII, as seen in Table 1. The FWHM of the Gaussian components were, in general, larger in the mutated PSII samples, with the widest peaks appearing in the G47 W PSII spectrum.

The relative amounts of the longer-wavelength component and shorter-wavelength component varied among the WT and mutated PSII samples, with the G47F PSII spectrum containing the most longer-wavelength component, the G47W spectrum containing the least longer-wavelength component, and the WT and T50F spectra containing a similar ratio to each other, as seen in Table 1; Figs. 5 and 6. In addition, in each PSII sample, the shorter-wavelength component of the Car∙+ peak decayed more quickly and to a larger extent. Therefore, there was a larger proportion of the longer-wavelength component present at longer times.

EPR Spectroscopy

Following the generation of YD, EPR spectra of WT, D2-T50F, D2-G47W, and D2-G47F PSII samples were collected in total darkness at 30 K, as seen in Fig. 7. The lineshapes vary slightly among the spectra. The spectra of T50F PSII grown at 10 μEinsteins/m2/s of illumination exhibit the most characteristic YD pattern. The WT spectrum also matches the lineshape reported in the literature for YD (Un et al. 1996; Tang et al. 1993; Noren et al. 1991). However, the spectra of PSII isolated from G47 W, T50F grown at 40 μEinsteins/m2/s of illumination, and G47F cells deviate increasingly from a normal YD spectrum. The shift could be due to a change in the orientation of the methylene protons with respect to the ring in the tyrosyl radicals of the mutated PSII samples, resulting in altered hyperfine coupling (Barry and Babcock 1998). However, due to the shift in g value of the baseline crossing point toward the free-electron g value and the consistency of the most upfield and downfield hyperfine peaks, it appears that the change in lineshape is due to an organic radical signal overlapping with YD. Although this is consistent with the presence of Chl∙+ and Car∙+, which may be generated by illumination, these species have a very short lifetime at 0 °C, and would have typically decayed during dark incubation. In addition, there is a larger amount of the organic radical signature present in the spectrum from T50F grown at 40 μEinsteins/m2/s of illumination than is present in the spectrum from T50F grown at 10 μEinsteins/m2/s of illumination, indicating that the presence of an overlapping radical EPR signal is due to an effect of high light during growth of the cells rather than an effect of the mutation on the structure of YD.

The samples containing YD were subsequently illuminated in the cryostat at 30 K for 60 min and spectra were recorded during the illumination, as seen in Figs. 8 and 9. During the illumination, Chl∙+ and Car∙+ (Figs. 8 and 9), which have indistinguishable g values at X band (Hanley et al. 1999), and some oxidized Cyt b559 (data not shown) were formed. For the WT PSII sample (Fig. 8A), the total yield of oxidized secondary donors was generated within 5 min of illumination. In contrast, in the G47F PSII sample (Fig. 8B), the maximum yield of oxidized secondary donors was not reached until after 30 min of illumination.

The procedure for generating YD results in one dark-stable YD per PSII, formed on ice before the sample is frozen for measurement of the EPR spectrum. Double integration of the YD EPR spectra gives the area corresponding to one radical per PSII center, once it has been corrected for any other overlapping organic radical signals. Under illumination at cryogenic temperatures, PSII is limited to one charge separation, and a second EPR signal is generated from the electron donor in each PSII center. Therefore, there should be a total of two oxidized species on the electron donor side of PSII for each PSII center: one YD and either Chl∙+, Car, Car∙+, or oxidized Cyt b559. However, the kinetics of formation of the second radical varied among the WT and mutated PSII samples, as seen in Fig. 9. WT and T50F samples generated the full radical yield within a few minutes of illumination. G47W samples took slightly longer to reach the total yield, while G47F samples reached two radicals per PSII only after a full 60 min of illumination (data from 30 to 60 min not shown).


CarD2 occupies a position between P680, the initial oxidant, and Cyt b559, the terminal electron donor, in the path of secondary electron transfer. Removing or disrupting this cofactor would be expected to alter the electron-transfer properties of PSII, if CarD2 is involved as an early donor in the secondary electron-transfer pathway. Indeed, the yields and kinetics of Car and Chl radical formation are altered in PSII samples that have been mutated to alter D2-G47 and D2-T50, two amino acids near CarD2. We have studied the properties of these mutated PSII complexes in which CarD2 is perturbed in order to gain more information on the secondary electron-transfer cofactors and their connectivity.

At cryogenic temperatures, illumination generates one stable charge separation per PSII, resulting in the formation of QA and either Car, Chl∙+, Car∙+, or oxidized Cyt b559. Cyt b559, which has the lowest reduction potential, is the preferred and terminal secondary electron donor within PSII. When Cyt b559 is preoxidized, one Car, Chl∙+, or Car∙+ intermediate is observed per PSII center upon illumination. The relative amounts of these radicals generated in a PSII sample are affected by temperature (Tracewell and Brudvig 2003) and by sample conditions (Hanley et al. 1999). If there were one accessible cofactor with the lowest reduction potential in each PSII center, a single radical would be generated, rather than a distribution. Therefore, the cofactors must be closely spaced in redox potential and have good connectivity to result in different radicals being trapped in different PSII centers. The D2-G47W, D2-G47F, and D2-T50F mutations are located near the headgroup of CarD2 and are expected to perturb CarD2 sterically, while the F and W residues may also participate in π stacking. These changes may affect the stability of CarD2∙+ relative to the other redox-active Car and Chl cofactors, because their redox potentials are so closely spaced.

It is known that there are at least two redox-active Car (Tracewell and Brudvig 2003; Telfer et al. 2003), and five redox-active Chl (Tracewell and Brudvig 2008) in the secondary electron-transfer pathways of PSII. However, the sequence of electron-transfer events and the specific identity of Car and Chl cofactors in the pathway are unknown (Faller et al. 2001). The effect of perturbing CarD2 on the rates and yields Chl∙+ and Car∙+ formation will depend on the connectivity of CarD2 with the other redox cofactors in the secondary electron-transfer pathway. For example, if another redox cofactor were capable of donating an electron to P680∙+ on an appropriate timescale, then the effect of perturbing CarD2 could be negligible. However, in each of the mutated PSII samples (D2-G47W, D2-G47F, and D2-T50F), a substantial decrease in yield of the secondary donors is observed by near-IR spectroscopy (Fig. 4A). Therefore, CarD2 seems to act as a bottleneck, resulting in decreased yield of the Car peak at 750 nm, the Chl∙+ peak from 800 to 840 nm, and the Car∙+ peak near 1,000 nm in all mutated PSII samples. Thus, there is no efficient alternative pathway for transferring electrons to P680∙+. Similarly, as observed by EPR spectroscopy around the g = 2 region, the kinetics of formation for the secondary donor radicals are much slower in the G47F and G47W-mutated PSII samples than in the WT sample, although they are comparable to WT in the T50F-mutated PSII sample, which was modeled as having the smallest perturbation to CarD2 (Fig. 9). The G47F and G47W-mutated PSII samples are less efficient at forming a charge separation between QA and the secondary donors, indicating that CarD2 is involved in this process. The decreased yield and impaired kinetics of the mutated PSII samples indicate that CarD2 is an early intermediate in secondary electron transfer, consistent with CarD2 being the initial electron donor to P680 and the initial step in an extended “branched” secondary electron-transfer pathway.

In addition to the decreased overall radical yield, there is a specific perturbation of the near-IR spectrum in each mutated PSII sample: the maximum of the Car∙+ peak is shifted to slightly longer wavelengths (Fig. 4B), while the maxima of the Chl∙+ and Car peaks remain unchanged. This indicates that the Car is not generated from CarD2, but most likely from a Car with a nearby proton accepting amino acid residue, as previously proposed (Gao et al. 2009). Furthermore, when the Car∙+ peak is deconvoluted into two Gaussian components, each corresponding to a redox-active Car∙+ (Tracewell and Brudvig 2003), the shorter-wavelength component shifts significantly more than the longer-wavelength component (more than three times, see Table 1). In WT PSII, the shorter-wavelength component has a maximum at 980 nm and a FWHM of 37.9 nm, and is the dominant contribution to the Car∙+ peak at 20 K. It decays much more quickly than the longer-wavelength component, although it remains the dominant contribution to the peak over 8 h of dark decay, indicating that it is closer to QA than is the other Car∙+. We assign this faster-decaying, shorter-wavelength component with a maximum at 980 nm to CarD2∙+. Although CarD2 has been proposed to be the initial electron donor in the pathway of secondary electron transfer (Lakshmi et al. 2003; Tracewell and Brudvig 2003), the specific spectral perturbations of site-directed mutations near CarD2 on the 980 nm Car∙+ species provide the first direct evidence that CarD2 is one of the redox-active Car in PSII.

Previous studies have shown that the maximum of the Car∙+ near-IR peak shifts to a slightly shorter wavelength when YD is oxidized to YD in all PSII centers (Tracewell and Brudvig 2003). It was hypothesized that this was either due to an electrochromic shift caused by YD or due to biasing electron transfer so that the redox-active Car closest to YD would remain reduced to avoid electrostatic repulsion. However, it has been observed that electrochromic shifts propagate substantial distances through PSII. For example, generating QA affects the visible spectrum of BA, the accessory Chl near PA of P680, from 21 Å away, and also possibly affects the spectrum of BB, 29 Å away (Stewart et al. 2000). Although YD would most likely have a smaller electrochromic effect than QA, its effects do propagate at least as far as P680 (Diner and Rappaport 2002). CarD2 is approximately 25 Å from YD. Alternatively, there are several Car cofactors in CP47 that are at a comparable or even shorter distance from YD; one Car in CP47 is 21 Å from YD, another is 27 Å away, and two others are about 30 Å from YD. Due to closely spaced distances, an electrochromic shift would not be a definitive indicator of which Car is oxidized, even if it were observable at those distances. It is also possible that oxidation of YD may bias the path of secondary electron transfer. To pull an electron from one of the Car in CP47, two intermediate Chl∙+ would be involved that are each 20 Å from YD, to ultimately generate a terminal Car∙+ that may be as close as 21 Å to YD. Under these conditions, the 980 nm CarD2∙+ may be a more stable radical than the 999 nm Car∙+, resulting in a net shift of the Car∙+ peak to a shorter wavelengths.

The near-IR spectra of D2-G47W, D2-G47F, and D2-T50F PSII samples contain a relatively larger amount of the Chl∙+ peak as compared to the Car∙+ peak than WT PSII samples (Fig. 4B). One possibility is that the mutations around the headgroup of CarD2 caused a shift of the reduction potential of CarD2∙+ to a higher value, making it more difficult to oxidize CarD2 relative to other Chl and Car cofactors. This would destabilize CarD2∙+, which is the predominant donor in the charge separation (980 nm Car∙+, see Fig. 5; Table 1), thus favoring Chl∙+ in a greater portion of PSII centers. This model can explain the observations for the G47F PSII sample, which has both a lower yield of CarD2∙+ relative to the other Car∙+ and also a higher yield of Chl∙+. Alternatively, the mutations around the headgroup of CarD2 and its change in conformation may have affected the distances to other cofactors, biasing the electron-transfer pathway in a different direction, such as towards CP47, which is adjacent to the mutations and contains an extended cluster of Chl relatively close to CarD2 (Fig. 2). This model can explain the observations for the G47W PSII sample, which has the largest relative amount of Chl∙+ and also has the most CarD2∙+ compared to the other Car∙+. It is likely that a combination of these factors occurs. Regardless, the relative Chl∙+ radical yield is higher in each of the mutated PSII samples.

The mutated PSII samples isolated from cells grown at higher light exhibit a dark-stable radical observed by EPR spectroscopy (Fig. 7). The dark-stable radical has the appearance of an organic radical, and could be either a Chl∙+ or Car∙+, although it is unusual in that it persists on ice for more than 2 min in the dark. However, a similar observation has been made for PSII samples subjected to photoinhibitory illumination (Blubaugh et al. 1991). The G47F PSII sample has the largest amount of the dark-stable radical, and it also has the slowest kinetics of charge separation. Therefore, it is possible that the dark-stable radical is associated with a quenching state, such that there is a decrease in the stability and efficiency of charge separation (Schweitzer and Brudvig 1997).

In addition, the shape of the Chl∙+ peak appears to depend on the light exposure during growth. The PSII samples isolated from G47W cells grown at 10 μEinsteins/m2/s, and from T50F cells grown at 10 μEinsteins/m2/s show a double Chl∙+ peak with maxima at 812 and 826 nm. Conversely, PSII isolated from G47F cells grown at 40 μEinsteins/m2/s and from T50F cells grown at 40 μEinsteins/m2/s only display one Chl∙+ peak. Moreover, the G47F and T50F PSII samples from cells grown under 40 μEinsteins/m2/s of illumination contain the largest amounts of the dark-stable radical. This suggests that the dark-stable radical may reflect a bias in the pathways of secondary electron transfer such that fewer Chl cofactors are oxidized in PSII samples isolated from cells grown under high light than those grown under lower light conditions. The Chl∙+ peak in WT PSII also appears to have only one peak, but it is broader than the single peak in T50F and G47F PSII samples. It seems that the double Chl∙+ peak is observed for cells grown under lower light. A double Chl∙+ peak has been previously observed for spinach PSII, but not for Synechocystis PCC 6803 PSII (Tracewell et al. 2001). Perhaps the double versus single Chl∙+ peak correlates in some way with photodamage and/or photoprotection, rather than an intrinsic species difference.

The Car∙+ near-IR absorption peak is wider in the mutated PSII samples relative to WT PSII samples, an indication that the Car∙+ population may have become less homogeneous as a result of the mutations. The G47W and T50F PSII samples have the widest Car∙+ peaks (Fig. 4). These wider peaks may be an indication that more than one longer-wavelength Car∙+ contributes to the peak; because the longer-wavelength Car∙+ arise from a charge separation that is more stable than that involving CarD2∙+, they would include components that are located further from QA than CarD2. Using high-frequency saturation-recovery EPR experiments, it has been found that the average distance from the nonheme iron to Car∙+ is 38 ± 1 Å (Lakshmi et al. 2003). Because CarD2∙+ is 36 Å from the nonheme iron, we can hypothesize that other candidate Car∙+ would be located about 40 Å from the nonheme iron. There are three Car molecules that are 40 Å from the nonheme iron: CarD1, a Car located at the interface of CP43 and PsbZ, and a Car located at the interface of CP47 and PsbM. There is previous evidence that ChlZD1, which is adjacent to CarD1, can be oxidized (Stewart et al. 1998). CarD1 oxidation is also observed in isolated PSII reaction centers, containing the subunits D1, D2, Cyt b559, and PsbI (Telfer et al. 1991). However, the two Car located at interfaces 40 Å from the nonheme iron are further from QA, and would, therefore, recombine more slowly than CarD2∙+, and are also located near lipids that may have an affect on their redox potential (Tracewell and Brudvig 2008). More evidence is required to identify the precise location of the longer-wavelength absorbing Car∙+. However, the shorter-wavelength Car∙+ component, with a maximum at 980 nm in WT, is CarD2∙+, as indicated by the significant shift of its wavelength maximum following a mutation around the headgroup of CarD2.

Electronic supplementary material


Guest Editor: Melvin Okamura


β-DM β-Dodecylmaltoside
Car β-Carotene
Car∙+ Oxidized Car
Chl Chlorophyll
Chl∙+ Oxidized Chl
CP47 47 kDa Chlorophyll-binding protein of photosystem II
Cyt b559 Cytochrome b559
D1/D2 Homologous PSII reaction center core proteins
DCMU 3-(3,4-Dichlorophenyl)-1,1-dimethylurea
EPR Electron paramagnetic resonance
FWHM Full width at half maximum
His-tagged PSII Synechocystis PCC 6803 PSII containing hexa-histidine-tagged CP47
MES 2-[N-morpholino] ethanesulfonic acid
OEC Oxygen-evolving complex
P680 Primary chlorophyll electron donor in PSII
Pheo Pheophytin
PSII Photosystem II
PCR Polymerase chain reaction
QA Non-exchanging plastoquinone electron acceptor in PSII
QB Exchangeable plastoquinone electron acceptor in PSII
WT Wild type
YZ/YD Redox-active tyrosines in the D1/D2 polypeptides

This study was supported by a grant from the DOE, Office of Basic Energy Sciences, Division of Chemical Sciences, DE-FG02-05ER15646 (G.W.B.), by a National Institutes of Health predoctoral traineeship, GM08283 (K.E.S.), and by the Engineering and Physical Sciences Research Council (EPSRC, EP/F00270X/1) and the Biotechnology and Biological Sciences Research Council (BBSRC, BB/C507037) (P.J.N.).

Barry BA,Babcock GT. Characterization of the tyrosine radical involved in photosynthetic oxygen evolutionChem ScrYear: 199828A117122
Bautista JA,Tracewell CA,Schlodder E,Cunningham FX,Brudvig GW,Diner BA. Construction and characterization of genetically modified Synechocystis sp. PCC 6803 photosystem II core complexes containing carotenoids with shorter π-conjugation than β-caroteneJ Biol ChemYear: 2005280388393885010.1074/jbc.M50495320016159754
Blubaugh DJ,Atamian M,Babcock GT,Golbeck JH,Cheniae GM. Photoinhibition of hydroxylamine-extracted photosystem II membranes: identification of the sites of photodamageBiochemistryYear: 1991307586759710.1021/bi00244a0301649634
Boehm M,Romero E,Reisinger V,Yu J,Komenda J,Eichacker LA,Dekker JP,Nixon PJ. Investigating the early stages of photosystem II assembly in Synechocystis sp. PCC 6803J Biol ChemYear: 2011286148121481910.1074/jbc.M110.20794421339295
Borg DC,Fajer J,Felton RH,Dolphin D. The π-cation radical of chlorophyll aProc Natl Acad Sci USAYear: 19706781382010.1073/pnas.67.2.81316591873
Buser CA,Diner BA,Brudvig GW. Photooxidation of cytochrome b559 in oxygen-evolving photosystem IIBiochemistryYear: 199231114491145910.1021/bi00161a0251445880
de Paula JC,Innes JB,Brudvig GW. Electron transfer in photosystem II at cryogenic temperaturesBiochemistryYear: 1985248114812010.1021/bi00348a0423004575
Diner BA,Rappaport F. Structure, dynamics, and energetics of the primary photochemistry of photosystem II of oxygenic photosynthesisAnnu Rev Plant BiolYear: 20025355158010.1146/annurev.arplant.53.100301.13523812221988
Emsley P,Cowtan K. Coot: model-building tools for molecular graphicsActa CrystallogrYear: 20046021262132
Faller P,Pascal A,Rutherford AW. β-Carotene redox reactions in photosystem II: electron transfer pathwayBiochemistryYear: 2001406431644010.1021/bi002602111371206
Gao Y,Shinopoulos KE,Tracewell CA,Focsan AL,Brudvig GW,Kispert LD. Formation of carotenoid neutral radicals in photosystem IIJ Phys Chem BYear: 20091139901990810.1021/jp807583219552399
Gerken S,Dekker JP,Schlodder E,Witt HT. Studies on the multiphasic charge recombination between chlorophyll aII+ (P-680+) and plastoquinone QA in photosystem II complexes. Ultraviolet difference spectrum of Chl-aII+/Chl-aIIBiochim Biophys Acta: BioenergeticsYear: 1989977526110.1016/S0005-2728(89)80008-8
Hanley J,Deligiannakis Y,Pascal A,Faller P,Rutherford AW. Carotenoid oxidation in photosystem IIBiochemistryYear: 1999388189819510.1021/bi990633u10387064
Holzwarth AR,Müller MG,Reus M,Nowaczyk M,Sander J,Rögner M. Kinetics and mechanism of electron transfer in intact photosystem II and in the isolated reaction center: pheophytin is the primary electron acceptorProc Natl Acad Sci USAYear: 20061036895690010.1073/pnas.050537110316641109
Kirilovsky D,Kerfeld CA. The orange carotenoid protein in photoprotection of photosystem II in cyanobacteriaBiochim Biophys Acta: BioenergeticsYear: 2012181715816610.1016/j.bbabio.2011.04.013
Lakshmi KV,Reifler MJ,Chisholm DA,Wang JY,Diner BA,Brudvig GW. Correlation of the cytochrome c550 content of cyanobacterial photosystem II with the EPR properties of the oxygen-evolving complexPhotosynth ResYear: 20027217518910.1023/A:101614090266216228516
Lakshmi KV,Poluektov OG,Reifler MJ,Wagner AM,Thurnauer MC,Brudvig GW. Pulsed high-frequency EPR study on the location of carotenoid and chlorophyll cation radicals in photosystem IIJ Am Chem SocYear: 20031255005501410.1021/ja029567112708850
Loll B,Kern J,Saenger W,Zouni A,Biesiadka J. Towards complete cofactor arrangement in the 3.0 Å resolution structure of photosystem IINatureYear: 20054381040104410.1038/nature0422416355230
Metz JG,Nixon PJ,Rogner M,Brudvig GW,Diner BA. Directed alteration of the D1 polypeptide of photosystem II: evidence that tyrosine-161 is the redox component, Z, connecting the oxygen-evolving complex to the primary electron donor, P680BiochemistryYear: 1989286960696910.1021/bi00443a0282510819
Nixon PJ, Boehm M, Michoux F, Yu J, Komenda J (2010) Recent advances in understanding the assembly and repair of Photosystem II. Ann Bot 106:1–16
Niyogi KK. Photoprotection revisited: genetic and molecular approachesAnnu Rev Plant PhysYear: 19995033335910.1146/annurev.arplant.50.1.333
Noren GH,Boerner RJ,Barry BA. EPR characterization of an oxygen-evolving photosystem II preparation from the transformable cyanobacterium Synechocystis 6803BiochemistryYear: 1991303943395010.1021/bi00230a0201850295
Rappaport F,Diner BA. Primary photochemistry and energetics leading to the oxidation of the (Mn)4Ca cluster and to the evolution of molecular oxygen in photosystem IICoordin Chem RevYear: 200825225927210.1016/j.ccr.2007.07.016
Reinman S,Mathis P,Conjeaud H,Stewart A. Kinetics of reduction of the primary donor of photosystem II. Influence of pH in various preparationsBiochim Biophys Acta: BioenergeticsYear: 198163542943310.1016/0005-2728(81)90040-2
Schweitzer RH,Brudvig GW. Fluorescence quenching by chlorophyll cations in photosystem IIBiochemistryYear: 199736113511135910.1021/bi97092039298954
Shinopoulos KE,Brudvig GW. Cytochrome b559 and cyclic electron transfer within photosystem IIBiochim Biophys Acta: BioenergeticsYear: 20121817667510.1016/j.bbabio.2011.08.002
Siegbahn PEM. O-O bond formation in the S4 state of the oxygen-evolving complex in photosystem IIChem Eur JYear: 2006129217922710.1002/chem.20060077417029313
Sproviero EM,Gascón JA,McEvoy JP,Brudvig GW,Batista VS. Computational studies of the O2-evolving complex of photosystem II and biomimetic oxomanganese complexesCoordin Chem RevYear: 200825239541510.1016/j.ccr.2007.09.006
Stewart DH,Brudvig GW. Cytochrome b559 of photosystem IIBiochim Biophys Acta: BioenergeticsYear: 19981367638710.1016/S0005-2728(98)00139-X
Stewart DH,Cua A,Chisholm DA,Diner BA,Bocian DF,Brudvig GW. Identification of histidine 118 in the D1 polypeptide of photosystem II as the axial ligand to chlorophyll ZBiochemistryYear: 199837100401004610.1021/bi980668e9665709
Stewart DH,Nixon PJ,Diner BA,Brudvig GW. Assignment of the Qy absorbance bands of photosystem II chromophores by low-temperature optical spectroscopy of wild-type and mutant reaction centersBiochemistryYear: 200039145831459410.1021/bi001246j11087414
Tan Q,Kuciauskas D,Lin S,Stone S,Moore AL,Moore TA,Gust D. Dynamics of photoinduced electron transfer in a carotenoid–porphyrin–dinitronaphthalenedicarboximide molecular triadJ Phys Chem BYear: 19971015214522310.1021/jp970682l
Tang XS,Chisholm DA,Dismukes GC,Brudvig GW,Diner BA. Spectroscopic evidence from site-directed mutants of Synechocystis PCC6803 in favor of a close interaction between histidine 189 and redox-active tyrosine 160, both of polypeptide D2 of the photosystem II reaction centerBiochemistryYear: 199332137421374810.1021/bi00212a0458257709
Telfer A,He W-Z,Barber J. Spectral resolution of more than one chlorophyll electron donor in the isolated photosystem II reaction centre complexBiochim Biophys Acta: BioenergeticsYear: 1990101714315110.1016/0005-2728(90)90145-T
Telfer A,De Las Rivas J,Barber J. β-Carotene within the isolated photosystem II reaction centre: photooxidation and irreversible bleaching of this chromophore by oxidised P680Biochim Biophys Acta: BioenergeticsYear: 1991106010611410.1016/S0005-2728(05)80125-2
Telfer A,Frolov D,Barber J,Robert B,Pascal A. Oxidation of the two β-carotene molecules in the photosystem II reaction centerBiochemistryYear: 2003421008101510.1021/bi026206p12549921
Thompson LK,Brudvig GW. Cytochrome b559 may function to protect photosystem II from photoinhibitionBiochemistryYear: 1988276653665810.1021/bi00418a0023058202
Thompson LK,Miller AF,Buser CA,de Paula JC,Brudvig GW. Characterization of the multiple forms of cytochrome b559 in photosystem IIBiochemistryYear: 1989288048805610.1021/bi00446a0122557895
Tracewell CA,Brudvig GW. Two redox-active β-carotene molecules in photosystem IIBiochemistryYear: 2003429127913610.1021/bi034584412885246
Tracewell CA,Brudvig GW. Multiple redox-active chlorophylls in the secondary electron-transfer pathways of oxygen-evolving photosystem IIBiochemistryYear: 200847115591157210.1021/bi801461d18850718
Tracewell CA,Cua A,Stewart DH,Bocian DF,Brudvig GW. Characterization of carotenoid and chlorophyll photooxidation in photosystem IIBiochemistryYear: 20014019320310.1021/bi001992o11141071
Umena Y,Kawakami K,Shen JR,Kamiya N. Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 ÅNatureYear: 2011473556010.1038/nature0991321499260
Un S,Tang XS,Diner BA. 245 GHz high-field EPR study of tyrosine-D° and tyrosine-Z° in mutants of photosystem IIBiochemistryYear: 19963567968410.1021/bi95237698547247
Vermeglio A,Mathis P. Light-induced absorbance changes at −170 °C with spinach chloroplasts: charge separation and field effectBiochim Biophys Acta: BioenergeticsYear: 197436891710.1016/0005-2728(74)90091-7
Vrettos JS,Stewart DH,de Paula JC,Brudvig GW. Low-temperature optical and resonance Raman spectra of a carotenoid cation radical in photosystem IIJ Phys Chem BYear: 19991036403640610.1021/jp991464q


[Figure ID: Fig1]
Fig. 1 

The arrangement of cofactors in the D1/D2/Cyt b559 sub-complex of cyanobacterial PSII, viewed along the membrane plane (PDB ID: 3ARC). Black arrows represent electron transfer. The oxygen-evolving complex (OEC) is shown with manganese ions in purple, oxygen in red, and calcium in green; tyrosine Z (YZ) and tyrosine D (YD) are shown in yellow; chlorophylls (Chl) are shown in green; β-carotenes (Car) are shown in orange; pheophytins (PheoA and PheoB) are shown in magenta; quinones (QA and QB) are shown in blue; and cytochrome b559 (Cyt b559) and the nonheme iron are shown in red. The surface of the protein is shown in the background and colored according to atom identity with C in green, N in blue, and O in red

[Figure ID: Fig2]
Fig. 2 

The arrangement of cofactors in PSII, viewed from the membrane surface (PDB ID: 3ARC). The oxygen-evolving complex (OEC) is shown with manganese atoms in purple, oxygen in red, and calcium in green; tyrosine Z (YZ) and tyrosine D (YD) are shown in yellow; chlorophylls (Chl) are shown in green; β-carotenes (Car) are shown in orange; pheophytins (PheoA and PheoB) are shown in magenta; quinones (QA and QB) are shown in blue; and cytochrome b559 (Cyt b559) and the nonheme iron are shown in red. The surface of the protein is shown in the background and colored according to atom identity with C in green, N in blue, and O in red

[Figure ID: Fig3]
Fig. 3 

Electron-transfer cofactors in photosystem II, viewed along the membrane plane (PDB ID: 2AXT). The oxygen-evolving complex (OEC) is shown with manganese atoms in purple and calcium in green; tyrosine Z (YZ) and tyrosine D (YD) are shown in yellow; chlorophylls (Chl) are shown in green; β-carotene (Car) is shown in orange; pheophytins (PheoA and PheoB) are shown in magenta; quinones (QA and QB) are shown in blue; and cytochrome b559 (Cyt b559) and the nonheme iron are shown in red. The surface of the protein is shown in the background and colored according to atom identity with C in green, N in blue, and O in red. Top A model of WT PSII structure, containing D2-G47 and D2-T50 modeled in stick form. Inset an enlarged picture of G47, T50, and the β-ionylidene ring of CarD2 with the surrounding residues shown as lines, colored according to atom identity. Bottom A model of D2-G47W, with G47W and T50 modeled in stick form. Inset an enlarged picture of G47W, T50, and the β-ionylidene ring of CarD2 with the surrounding residues shown as lines, colored according to atom identity

[Figure ID: Fig4]
Fig. 4 

Light-minus-dark near-IR spectra of Synechocystis PSII samples from WT cells grown under 40 μEinsteins/m2/s of illumination (black), T50F cells grown under 10 μEinsteins/m2/s of illumination (green), T50F cells grown under 40 μEinsteins/m2/s (orange), G47W cells grown under 10 μEinsteins/m2/s of illumination (red), and G47F cells grown under 40 μEinsteins/m2/s of illumination (blue), recorded at 20 K. A Normalized to equal chlorophyll concentration. B Each Car∙+ peak normalized to 1. C Each Chl∙+ peak normalized to 1

[Figure ID: Fig5]
Fig. 5 

Gaussian deconvolutions of the Car∙+ peak formed by illumination for 15 min at 20 K. A The WT PSII difference spectrum after 0 min of dark incubation. B The WT PSII difference spectrum after 3 h of dark incubation. C The G47W PSII difference spectrum after 0 min of dark incubation. D The G47W PSII difference spectrum after 3 h of dark incubation. The two Gaussian components from Table 1 are shown in blue (shorter-wavelength component) and green (longer-wavelength component), their sum is shown in red, and the raw data are shown in black

[Figure ID: Fig6]
Fig. 6 

The decay in absorbance, as a function of dark incubation time, of the shorter-wavelength component (blue) and the longer-wavelength component (green). A WT PSII samples. B T50F PSII samples. C G47W PSII samples. D G47F PSII samples

[Figure ID: Fig7]
Fig. 7 

EPR spectra in the YD region of PSII isolated from WT cells grown under 40 μEinsteins/m2/s of illumination (black), T50F cells grown under 10 μEinsteins/m2/s of illumination (green), T50F cells grown under 40 μEinsteins/m2/s (orange), G47W cells grown under 40 μEinsteins/m2/s of illumination (red), and G47F cells grown under 40 μEinsteins/m2/s of illumination (blue). Instrument settings:  temperature, 30 K; microwave power, 105 μW; and field modulation amplitude, 4 G

[Figure ID: Fig8]
Fig. 8 

The EPR spectra collected as samples were illuminated in the cryostat with a xenon lamp for 1 h. A WT spectra collected in the dark (black) and after 0 (red), 5 (green), 10 (blue), 15 (red), 20 (green), 25 (blue), 30 (blue), 35 (red), 40 (green), 45 (blue), 50 (red), 55 (green), and 60 (blue) minutes of illumination. B G47F spectra collected in the dark (black) and after 2 (red), 8 (green), 12 (blue), 17 (red), 22 (green), 25 (blue), 30 (red), 34 (green), 38 (blue), 42 (red), 47 (green), 51 (blue), 55 (red), and 60 (green) minutes of illumination. Instrument settings as in Fig. 7

[Figure ID: Fig9]
Fig. 9 

The radical yield per PSII as a function of illumination time, obtained by double integration of the EPR spectra of WT (black), T50F (green), G47W (red), and G47F (blue) PSII samples, recorded at 30 K. Instrument settings as in Fig. 7

[TableWrap ID: Tab1] Table 1 

The peak parameters of the two Gaussian components of the Car∙+ peak present in WT, T50F, G47F, and G47W PSII samples

λ1 (nm) Initial % FWHM1 (nm) λ2 (nm) Initial % FWHM2 (nm)
WT 980.4 69 37.9 999.2 31 74.1
T50F 989.3 68 43.2 999.8 32 92.8
G47F 988.3 48 40.8 1001 52 68.0
G47W 993.3 82 55.0 1003 17 127

Article Categories:
  • Regular Paper

Keywords: Keywords β-Carotene radical, Chlorophyll radical, Cytochrome b559, EPR spectroscopy, Near-IR spectroscopy, Photosystem II, Site-directed mutagenesis.

Previous Document:  Intention to quit smoking and concerns about household environmental risks: findings from the Health...
Next Document:  Combination analysis of Bub1 and Mad2 expression in endometrial cancer: act as a prognostic factor i...