Document Detail

Using a quality control approach to define an 'adequately cellular' liquid-based cervical cytology specimen.
MedLine Citation:
PMID:  16879263     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To define a minimum acceptable total squamous cellularity for (ThinPrep) liquid-based cervical cytology (LBC) specimens using quality control techniques. METHODS: Two hundred LBC preparations were made containing varying numbers (<200) of severely dyskaryotic squamous cells and with varying total cellularities. RESULTS: Ninety-eight per cent of the LBC preparations that were missed by one or more of three cytoscreeners had fewer than 16 abnormal objects (single dyskaryotic cells or clumps of cells) and 87 dyskaryotic cells. The minimum ratio of dyskaryotic to total squamous cells that, in a preparation of 5000 squamous cells has a probability of at least 0.98 that 87 or more dyskaryotic cells will be present is 1:47. Twenty-three preparations diagnosed as abnormal had ratios of dyskaryotic to total squamous cells of between 1:2.5 and 1:4596. There is thus no feasible minimum acceptable squamous cellularity that will give an acceptable probability of detection of all specimen vials containing abnormal cells in the observed proportions. CONCLUSIONS: It is suggested that the minimum acceptable cellularity for LBC specimens is set pragmatically by the screening programme to give a feasible percentage of repeat tests.
F McQueen; E Duvall
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytopathology : official journal of the British Society for Clinical Cytology     Volume:  17     ISSN:  0956-5507     ISO Abbreviation:  Cytopathology     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-01     Completed Date:  2006-09-15     Revised Date:  2007-11-13    
Medline Journal Info:
Nlm Unique ID:  9010345     Medline TA:  Cytopathology     Country:  England    
Other Details:
Languages:  eng     Pagination:  168-74     Citation Subset:  IM    
Pathology Department, Royal Infirmary of Edinburgh, Edinburgh, UK.
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MeSH Terms
Carcinoma, Squamous Cell / diagnosis*
False Negative Reactions
Quality Control
Reproducibility of Results
Sensitivity and Specificity
Uterine Cervical Neoplasms / diagnosis*
Vaginal Smears* / methods,  standards
Comment In:
Cytopathology. 2007 Oct;18(5):325-6; author reply 326   [PMID:  17883693 ]
Cytopathology. 2006 Aug;17(4):165-7   [PMID:  16879262 ]

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