| Using proteomic analysis of the human amniotic fluid to identify histologic chorioamnionitis. | |
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MedLine Citation:
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PMID: 18238979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To estimate the relationship between histologic chorioamnionitis and four amniotic fluid proteomic biomarkers characteristic of inflammation (defensins 2 and 1, calgranulins C and A). METHODS: One hundred fifty-eight women with singleton pregnancies had a clinically indicated amniocentesis to rule out inflammation and infection in the context of preterm labor or preterm premature rupture of membranes. A proteomic fingerprint (Mass Restricted score) was generated from amniotic fluid using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. The Mass Restricted score ranges from 0 to 4 (none to all four biomarkers present) in direct relationship with severity of intra-amniotic inflammation. Presence or absence of biomarkers was analyzed in relationship to placental pathology. Criteria for severity of histologic chorioamnionitis were 3 stages and 4 grades of inflammation of the amnion, choriodecidua and chorionic plate. RESULTS: The prevalence of histologic chorioamnionitis was 64% (stage I 12%, stage II 16%, and stage III 37%). The Mass Restricted score significantly correlated with stages of histologic chorioamnionitis (r=0.539, P<.001), grades of choriodeciduitis (r=0.465, P<.001), and amnionitis (r=0.536, P<.001). African-American women were overrepresented in the group with severe inflammation (Mass Restricted score 3-4, P=.022). Of the four biomarkers of the Mass Restricted score, calgranulin C had the strongest relationship with presence of stage III chorioamnionitis, independent of race, amniocentesis-to-delivery interval, and gestational age. CONCLUSION: Proteomic analysis of amniotic fluid provides an opportunity for early recognition of histologic chorioamnionitis. This methodology may in the future identify candidates for antenatal therapeutic interventions. LEVEL OF EVIDENCE: II. |
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Authors:
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Irina A Buhimschi; Eduardo Zambrano; Christian M Pettker; Mert Ozan Bahtiyar; Michael Paidas; Victor A Rosenberg; Stephen Thung; Carolyn M Salafia; Catalin S Buhimschi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Obstetrics and gynecology Volume: 111 ISSN: 0029-7844 ISO Abbreviation: Obstet Gynecol Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-01 Completed Date: 2008-03-17 Revised Date: 2009-10-26 |
Medline Journal Info:
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Nlm Unique ID: 0401101 Medline TA: Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 403-12 Citation Subset: AIM; IM |
Affiliation:
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Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520, USA. irina.buhimschi@yale.edu |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult African Americans Amniocentesis / methods Amniotic Fluid / chemistry* Biological Markers / analysis Chorioamnionitis / diagnosis*, epidemiology, metabolism European Continental Ancestry Group Female Humans Inflammation Pregnancy Pregnancy Complications, Infectious / diagnosis* Pregnancy Outcome Prevalence Proteome / analysis* Proteomics / methods* Reproducibility of Results Sensitivity and Specificity Severity of Illness Index Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Grant Support | |
ID/Acronym/Agency:
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R01 HD 047321-01/HD/NICHD NIH HHS; R01 HD047321-01/HD/NICHD NIH HHS; R01 HD047321-02/HD/NICHD NIH HHS; R01 HD047321-03/HD/NICHD NIH HHS; R01 HD047321-04/HD/NICHD NIH HHS; R01 HD047321-05/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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