Document Detail

Using proteomic analysis of the human amniotic fluid to identify histologic chorioamnionitis.
MedLine Citation:
PMID:  18238979     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To estimate the relationship between histologic chorioamnionitis and four amniotic fluid proteomic biomarkers characteristic of inflammation (defensins 2 and 1, calgranulins C and A).
METHODS: One hundred fifty-eight women with singleton pregnancies had a clinically indicated amniocentesis to rule out inflammation and infection in the context of preterm labor or preterm premature rupture of membranes. A proteomic fingerprint (Mass Restricted score) was generated from amniotic fluid using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. The Mass Restricted score ranges from 0 to 4 (none to all four biomarkers present) in direct relationship with severity of intra-amniotic inflammation. Presence or absence of biomarkers was analyzed in relationship to placental pathology. Criteria for severity of histologic chorioamnionitis were 3 stages and 4 grades of inflammation of the amnion, choriodecidua and chorionic plate.
RESULTS: The prevalence of histologic chorioamnionitis was 64% (stage I 12%, stage II 16%, and stage III 37%). The Mass Restricted score significantly correlated with stages of histologic chorioamnionitis (r=0.539, P<.001), grades of choriodeciduitis (r=0.465, P<.001), and amnionitis (r=0.536, P<.001). African-American women were overrepresented in the group with severe inflammation (Mass Restricted score 3-4, P=.022). Of the four biomarkers of the Mass Restricted score, calgranulin C had the strongest relationship with presence of stage III chorioamnionitis, independent of race, amniocentesis-to-delivery interval, and gestational age.
CONCLUSION: Proteomic analysis of amniotic fluid provides an opportunity for early recognition of histologic chorioamnionitis. This methodology may in the future identify candidates for antenatal therapeutic interventions.
Irina A Buhimschi; Eduardo Zambrano; Christian M Pettker; Mert Ozan Bahtiyar; Michael Paidas; Victor A Rosenberg; Stephen Thung; Carolyn M Salafia; Catalin S Buhimschi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Obstetrics and gynecology     Volume:  111     ISSN:  0029-7844     ISO Abbreviation:  Obstet Gynecol     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-01     Completed Date:  2008-03-17     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0401101     Medline TA:  Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  403-12     Citation Subset:  AIM; IM    
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MeSH Terms
African Americans
Amniocentesis / methods
Amniotic Fluid / chemistry*
Biological Markers / analysis
Chorioamnionitis / diagnosis*,  epidemiology,  metabolism
European Continental Ancestry Group
Pregnancy Complications, Infectious / diagnosis*
Pregnancy Outcome
Proteome / analysis*
Proteomics / methods*
Reproducibility of Results
Sensitivity and Specificity
Severity of Illness Index
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Grant Support
R01 HD 047321-01/HD/NICHD NIH HHS; R01 HD047321/HD/NICHD NIH HHS; R01 HD047321-01/HD/NICHD NIH HHS; R01 HD047321-02/HD/NICHD NIH HHS; R01 HD047321-03/HD/NICHD NIH HHS; R01 HD047321-04/HD/NICHD NIH HHS; R01 HD047321-05/HD/NICHD NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/Proteome

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