Document Detail

Using poly(lactide-co-glycolide) electrospun scaffolds to deliver cultured epithelial cells to the cornea.
MedLine Citation:
PMID:  20455650     Owner:  NLM     Status:  MEDLINE    
AIMS: To assess the potential of electrospun poly(lactide-co-glycolide) membranes to provide a biodegradable cell carrier system for limbal epithelial cells. MATERIAL & METHODS: 50:50 poly(lactide-co-glycolide) scaffolds were spun, sterilized and seeded with primary rabbit limbal epithelial cells. Cells were cultured on the scaffolds for 2 weeks and then examined by confocal microscopy, cryosectioning and scanning-electron microscopy. The tensile strength of scaffolds before and after annealing and sterilization was also studied. RESULTS: The limbal cells had formed a continuous multilayer of cells on either side of the scaffold. Scaffolds with cells showed signs of the onset of degradation within 2 weeks in culture media at 37 degrees C. Scaffolds that were annealed resulted in a more brittle and stiff mat. CONCLUSIONS: We suggest this carrier membrane could be used as a replacement for the human amniotic membrane in the treatment of limbal stem cell deficiency, lowering the risk of disease transmission to the patient.
Pallavi Deshpande; Rob McKean; Keith A Blackwood; Richard A Senior; Adekemi Ogunbanjo; Anthony J Ryan; Sheila MacNeil
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Regenerative medicine     Volume:  5     ISSN:  1746-076X     ISO Abbreviation:  Regen Med     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-11     Completed Date:  2010-10-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101278116     Medline TA:  Regen Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  395-401     Citation Subset:  IM    
Department of Engineering Materials, Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield, S3 7HQ, UK.
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MeSH Terms
Cell Culture Techniques
Cells, Cultured / cytology
Cornea / cytology*
Epithelial Cells / cytology*,  transplantation
Microscopy, Confocal / methods
Microscopy, Electron, Scanning / methods
Polyglactin 910 / chemistry*
Regenerative Medicine / methods*
Time Factors
Tissue Engineering / methods
Tissue Scaffolds
Reg. No./Substance:
34346-01-5/Polyglactin 910

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