Document Detail


Using point-of-care CD34 enumeration to optimize PBSC collection conditions.
MedLine Citation:
PMID:  12028839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A PBSC graft containing 4-5 x 10(6) CD34(+) cells/kg is considered optimal in terms of durable engraftment. Tracking CD34 kinetics via point-of-care testing during PBSC mobilization could determine which (and when) patients will yield an optimal product. We evaluated whether microvolume fluorimetry (MVF) would be useful in optimizing PBSC mobilization/harvest and if it will shorten our standard 6 h collection. METHODS: Absolute CD34 values were obtained using the IMAGN 2000 and STELLer CD34 assay (50 microL sample volume). Peripheral blood (PB) CD34 values from 30 patients undergoing PBSC mobilization were used to generate a PB CD34-based algorithm that would predict collection day/duration of apheresis. The algorithm was then used prospectively to collect PBSC products on 50 hematologic malignancy (HM) patients. RESULTS: Using the algorithm, patients were assigned to either a 6 (11-20 CD34/microL), 4 (21-49 CD34/microL) or 2 (> or = 50 CD34/microL) h collection. Patients with a CD34 value < or = 10/microL were re-tested. All patients (n = 43) predicted to mobilize reached the optimal CD34 (4-5 x 10(6)/kg) value with 1.0 apheresis procedure; seven patients had < or = 10/microL (nonmobilizers). The majority (75%) had apheresis charges decreased by 33-66%; 47% only required a 2 h procedure and 28% required 4 h. All patients demonstrated rapid trilineage engraftment. DISCUSSION: Absolute PB CD34 measurement using MVF offers a rapid and reliable approach to obtaining optimal PBSC products with minimal technical expertise. Although not a replacement for conventional flow cytometry, it meets the requirements for a point-of-care procedure.
Authors:
S J Noga; G B Vogelsang; S C Miller; S Meusel; K Loper; R Case; B Myers; L Rogers; I Flinn; M Borowitz; P O'Donnell
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytotherapy     Volume:  3     ISSN:  1465-3249     ISO Abbreviation:  Cytotherapy     Publication Date:  2001  
Date Detail:
Created Date:  2002-05-24     Completed Date:  2003-01-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100895309     Medline TA:  Cytotherapy     Country:  England    
Other Details:
Languages:  eng     Pagination:  11-8     Citation Subset:  IM    
Affiliation:
The Johns Hopkins Hematopoietic and Therapeutic Support Service, Baltimore, MD 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Antigens, CD34 / analysis*
Blood Component Removal / methods*
Erythroid Precursor Cells / transplantation*
Fluorometry / methods
Hematologic Neoplasms / blood,  therapy
Hematopoietic Stem Cell Mobilization / methods*
Humans
Point-of-Care Systems*
Time Factors
Treatment Outcome
Grant Support
ID/Acronym/Agency:
CA06973/CA/NCI NIH HHS; CA15396/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD34

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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