| Using paraquat to generate anion free radicals and hydrogen peroxide in in vitro: Antioxidant effect of vitamin E: A procedure to teach theoretical and experimental principles of reactive oxygen species biochemistry. | |
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MedLine Citation:
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PMID: 21567804 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The theoretical basis of reactive oxygen species and their impact on health issues are relatively easy to understand by biomedical students. The detection of reactive oxygen species requires expensive equipment, the procedures are time consuming and costly, and the results are hard to interpret. Moreover, cause-and-effect relationships in the living system are not so evident. In this report, we adapted a two-step procedure to detect anion superoxide radicals and hydrogen peroxide generation in lymphocytes exposed to paraquat by using nitroblue tetrazolium salt and dihydrorhodamine, respectively. Also, a two-step assay was performed to evaluate lymphocyte viability and nuclei morphologic changes on paraquat exposure for 1 and 24 hours incubation time by using trypan blue exclusion assay and acridine orange/ethidium bromide staining technique, respectively. Vitamin E was used as antioxidant to inhibit the deleterious effects of paraquat on cells. Students learned how to (i) design and perform experiments in the laboratory, (ii) read critical scientific literature, and (iii) discuss and contrast relevant information about reactive oxygen species as causative agents of cell death phenomenon. |
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Authors:
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Marlene Jimenez-Del-Rio; Gerson Suarez-Cedeño; Carlos Velez-Pardo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology Volume: 38 ISSN: 1539-3429 ISO Abbreviation: Biochem Mol Biol Educ Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2011-05-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100970605 Medline TA: Biochem Mol Biol Educ Country: United States |
Other Details:
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Languages: eng Pagination: 104-9 Citation Subset: - |
Copyright Information:
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Copyright © 2010 International Union of Biochemistry and Molecular Biology, Inc. |
Affiliation:
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School of Medicine, Medical Research Institute, Neuroscience Research Group, University of Antioquia (UdeA), Calle 62 # 52-59, Building 1, Laboratory 411/ 412; SIU- Medellin, Colombia. marlene.jimenez@neurociencias.udea.edu.co. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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