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Using a novel macro in vitro technique to estimate differences in absorption rates of volatile fatty acids in the rumen.
MedLine Citation:
PMID:  20487097     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A novel macro in vitro system was used to test the theory that rumen proportions of acetate, propionate and butyrate are not representative of their respective net production rates. Whole rumen content (10-16 kg) from two cows was mixed with a bicarbonate buffer and incubated separately in two 40-l in vitro vessels for 3 h. A total of six experimental periods were used. In this study, a total of six cows were used and fed 1/8 of the daily ration by hand every 3 h. To obtain differences in rumen volatile fatty acids (VFA) composition, 1 l of acetate (416 mm), propionate (108 mm), butyrate (79 mm), lactic acid (300 mm) or nothing was infused during 24 h into the rumen before collection of representative samples of rumen contents. Infusions of acids were then continued during the in vitro incubations in exact proportion to the digesta removed from the rumen. In Periods 1 and 2, the cows were alternatively infused with acetate or nothing. In Periods 3 and 4, the infusions consisted of propionate or butyrate and in Periods 5 and 6 of lactate or nothing. Nine liquid samples were obtained between 3 and 180 min after the start of incubation and analysed for concentrations of VFA. Changes in proportions of individual VFA were estimated by linear regression. No differences in VFA proportions were observed in the absence of infusion (p> 0.5) over time, but when individual VFA were infused, their respective proportions increased. This was interpreted as the result of a decreased in vitro fermentation rate of digesta substrates compared with that in the rumen. Lactate infusion increased butyrate proportion in vitro. It is concluded that this study could not provide any evidence that ruminal VFA proportions are unrepresentative of the proportions of net production.
Authors:
P Udén
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal physiology and animal nutrition     Volume:  95     ISSN:  1439-0396     ISO Abbreviation:  J Anim Physiol Anim Nutr (Berl)     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101126979     Medline TA:  J Anim Physiol Anim Nutr (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal of Animal Physiology and Animal Nutrition © 2010 Blackwell Verlag GmbH.
Affiliation:
Department of Animal Nutrition & Management, Kungsängen Research Centre, Swedish University of Agricultural Sciences, Uppsala, Sweden. peter.uden@huv.slu.se
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