Document Detail


Using label-free screening technology to improve efficiency in drug discovery.
MedLine Citation:
PMID:  22468914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Screening assays have traditionally utilized reporter labels to quantify biological responses relevant to the disease state of interest. However, there are limitations associated with the use of labels that may be overcome with temporal measurements possible with label-free.
AREAS COVERED: This review comprises general and system-specific information from literature searches using PubMed, published books and the authors' personal experience. This review highlights the label-free approaches in the context of various applications. The authors also note technical issues relevant to the development of label-free assays and their application to HTS.
EXPERT OPINION: The limitations associated with the use of transfected cell lines and the use of label-based assays are gradually being realized. As such, greater emphasis is being placed on label-free biophysical techniques using native cell lines. The introduction of 96- and 384-well plate label-free systems is helping to broker a wider acceptance of these approaches in high-throughput screening. However, potential users of the technologies remain skeptical, primarily because the physical basis of the signals generated, and their contextual relevance to cell biology and signal transduction, has not been fully elucidated. Until this is done, these new technology platforms are more likely to complement, rather than replace, traditional screening platforms.
Authors:
Reena Halai; Matthew A Cooper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2012-01-04
Journal Detail:
Title:  Expert opinion on drug discovery     Volume:  7     ISSN:  1746-045X     ISO Abbreviation:  Expert Opin Drug Discov     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-04-03     Completed Date:  2012-09-24     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  101295755     Medline TA:  Expert Opin Drug Discov     Country:  England    
Other Details:
Languages:  eng     Pagination:  123-31     Citation Subset:  IM    
Affiliation:
The University of Queensland, Institute for Molecular Bioscience, Brisbane, Qld, 4072, Australia.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Drug Design
Drug Discovery / methods*
High-Throughput Screening Assays / methods*
Humans
Staining and Labeling
Technology, Pharmaceutical / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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