Document Detail


Using intravital microscopy to observe bevacizumab-mediated anti-angiogenesis in human head and neck squamous cell carcinoma xenografts.
MedLine Citation:
PMID:  23039202     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONCLUSION: The study showed the value of using intravital microscopy (IVM) analysis for the study of neoangiogenesis. It demonstrated that the model and the analytical methodology could be used to evaluate in detail the effects of treatment strategies for solid tumours.
OBJECTIVES: Neoangiogenesis is a key component of tumour progression, invasion and metastasis. In clinical trials monoclonal antibodies specific for vascular endothelial growth factor - VEGF (bevacizumab) - have been shown to significantly affect tumour progression when given in combination with standard chemotherapy, and also to improve the overall survival of patients. For squamous cell carcinoma of the head and neck (HNSCC), we still await definitive evidence of the effect of such treatment. The present study was designed to investigate the anti-angiogenesis effect of beviacizumab in green fluorescent protein (GFP)-labelled HNSCC xenografts using IVM technology.
METHODS: We performed IVM and used image analysis for quantification of angiogenesis and of effects of bevacizumab on cell viability, combined with histochemical and immunohistochemical analysis to standardize the digital analysis of changes in tumour vascularization and cell viability.
RESULTS: We found significant effects of bevacizumab on angiogenesis and cancer cell survival in HNSCC. Repeated injections of bevacizumab were found to provide the greatest effects.
Authors:
Takashi Yamatodani; Bo Holmqvist; Elisabeth Kjellén; Anders Johnsson; Hiroyuki Mineta; Johan Wennerberg
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-07
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  132     ISSN:  1651-2251     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-20     Completed Date:  2013-05-17     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1324-33     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology/Head and Neck Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
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MeSH Terms
Descriptor/Qualifier:
Angiogenesis Inhibitors / pharmacology*
Animals
Antibodies, Monoclonal, Humanized / pharmacology*
Carcinoma, Squamous Cell / blood supply*
Cell Line, Tumor
Dermoscopy / methods*
Disease Models, Animal*
Humans
Mice
Mice, Nude
Neoplasm Transplantation / pathology*
Neovascularization, Pathologic / pathology*
Otorhinolaryngologic Neoplasms / blood supply*
Skin Window Technique*
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/Angiogenesis Inhibitors; 0/Antibodies, Monoclonal, Humanized; 2S9ZZM9Q9V/bevacizumab

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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