| Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailability. | |
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MedLine Citation:
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PMID: 20181868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Iron fortification of foods and biofortification of staple food crops are strategies that can help to alleviate Fe deficiency. The broiler chicken may be a useful model for initial in vivo screening of Fe bioavailability in foods due to its growth rate, anatomy, size, and low cost. In this study, we assess the broiler as a model for hemoglobin (Hb) maintenance studies and present a unique duodenal loop technique for direct measurement of intestinal Fe absorption. One-week-old chicks were allocated into Fe-deficient versus Fe-adequate treatment groups. For 6 wk, blood Hb, feed consumption, and BW were measured. At wk 7, birds were anesthetized and their duodenal loops were exposed. The loop was isolated and a nonocclusive catheter was inserted into the duodenal vein for blood sampling. A stable isotope solution containing (58)Fe (1 mg of Fe in 10 mM ascorbic acid) was injected into the loop. Blood samples were collected every 5 min and for 120 min postinjection and analyzed by inductively coupled argon-plasma mass spectrometry for (58)Fe concentrations. In the low-Fe group, Hb concentrations, total body Hb Fe, and BW were lower and Hb maintenance efficiency (indicator for dietary Fe availability) was higher than in the high-Fe group (P < 0.05). Iron absorption was higher in the Fe-deficient birds (P < 0.05). In addition, expression of proteins involved in Fe uptake and transfer [i.e., divalent metal transporter 1 (Fe uptake transporter), ferroportin (involved in Fe transport across the enterocyte), and duodenal cytochrome B reductase (reduces Fe at brush border membrane)] were elevated in the low-Fe group. These results indicate that this model exhibits the appropriate responses to Fe deficiency and has potential to serve as a model for Fe bioavailability. Such a model should be most useful as an intermediate test of in vivo Fe bioavailability observations in preparation for subsequent human studies. |
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Authors:
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E Tako; M A Rutzke; R P Glahn |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Poultry science Volume: 89 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-25 Completed Date: 2010-04-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: United States |
Other Details:
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Languages: eng Pagination: 514-21 Citation Subset: IM |
Affiliation:
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Department of Science, Cornell University, Ithaca, NY 14853, USA. et79@cornell.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorptiometry, Photon Animals Biological Availability* Caco-2 Cells Cation Transport Proteins / genetics, metabolism Chickens* / growth & development Cytochrome b Group / genetics, metabolism DNA, Complementary / genetics, metabolism Food Analysis Gene Expression Regulation / physiology Hemoglobins Humans Intestine, Small / metabolism Iron / administration & dosage, pharmacokinetics* |
| Chemical | |
Reg. No./Substance:
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0/Cation Transport Proteins; 0/Cytochrome b Group; 0/DNA, Complementary; 0/Hemoglobins; 0/metal transporting protein 1; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 7439-89-6/Iron |
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