Document Detail


Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailability.
MedLine Citation:
PMID:  20181868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Iron fortification of foods and biofortification of staple food crops are strategies that can help to alleviate Fe deficiency. The broiler chicken may be a useful model for initial in vivo screening of Fe bioavailability in foods due to its growth rate, anatomy, size, and low cost. In this study, we assess the broiler as a model for hemoglobin (Hb) maintenance studies and present a unique duodenal loop technique for direct measurement of intestinal Fe absorption. One-week-old chicks were allocated into Fe-deficient versus Fe-adequate treatment groups. For 6 wk, blood Hb, feed consumption, and BW were measured. At wk 7, birds were anesthetized and their duodenal loops were exposed. The loop was isolated and a nonocclusive catheter was inserted into the duodenal vein for blood sampling. A stable isotope solution containing (58)Fe (1 mg of Fe in 10 mM ascorbic acid) was injected into the loop. Blood samples were collected every 5 min and for 120 min postinjection and analyzed by inductively coupled argon-plasma mass spectrometry for (58)Fe concentrations. In the low-Fe group, Hb concentrations, total body Hb Fe, and BW were lower and Hb maintenance efficiency (indicator for dietary Fe availability) was higher than in the high-Fe group (P < 0.05). Iron absorption was higher in the Fe-deficient birds (P < 0.05). In addition, expression of proteins involved in Fe uptake and transfer [i.e., divalent metal transporter 1 (Fe uptake transporter), ferroportin (involved in Fe transport across the enterocyte), and duodenal cytochrome B reductase (reduces Fe at brush border membrane)] were elevated in the low-Fe group. These results indicate that this model exhibits the appropriate responses to Fe deficiency and has potential to serve as a model for Fe bioavailability. Such a model should be most useful as an intermediate test of in vivo Fe bioavailability observations in preparation for subsequent human studies.
Authors:
E Tako; M A Rutzke; R P Glahn
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Poultry science     Volume:  89     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-25     Completed Date:  2010-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  514-21     Citation Subset:  IM    
Affiliation:
Department of Science, Cornell University, Ithaca, NY 14853, USA. et79@cornell.edu
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MeSH Terms
Descriptor/Qualifier:
Absorptiometry, Photon
Animals
Biological Availability*
Caco-2 Cells
Cation Transport Proteins / genetics,  metabolism
Chickens* / growth & development
Cytochrome b Group / genetics,  metabolism
DNA, Complementary / genetics,  metabolism
Food Analysis
Gene Expression Regulation / physiology
Hemoglobins
Humans
Intestine, Small / metabolism
Iron / administration & dosage,  pharmacokinetics*
Chemical
Reg. No./Substance:
0/Cation Transport Proteins; 0/Cytochrome b Group; 0/DNA, Complementary; 0/Hemoglobins; 0/metal transporting protein 1; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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