| Using cell transplantation to investigate genes involved in aging. | |
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MedLine Citation:
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PMID: 12618009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell transplantation provides a way to study genes that may be important in human tissue aging. Studies on gene action in human cells are usually restricted to cell culture investigations and clinical observations. Differences in human and rodent cellular biology, particularly with respect to telomere dynamics, show the need for new systems for investigating aging that use human cells or cells of other large, long-lived mammals, such as bovine cells. The system we describe uses human and bovine adrenocortical cells transplanted into scid (severe combined immunodeficiency) mice. They form a vascularized tissue structure that can replace the essential functions of the animals' own adrenal glands. The cells may be genetically modified before introduction into the animal. Using hTERT (telomerase reverse transcriptase) and oncoproteins, we show the potential for investigating gene action in genetically modified tissues created by cell transplantation. |
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Authors:
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Peter J Hornsby; Meizhen Chen; Christina L Hawks; Qin Huang; Beicheng Sun; Lishan Wang; Michael Thomas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
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Title: Mechanisms of ageing and development Volume: 124 ISSN: 0047-6374 ISO Abbreviation: Mech. Ageing Dev. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-03-05 Completed Date: 2003-10-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0347227 Medline TA: Mech Ageing Dev Country: Ireland |
Other Details:
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Languages: eng Pagination: 79-84 Citation Subset: IM |
Affiliation:
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Department of Physiology and Sam and Ann Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center, 15355 Lambda Drive STCBM 2.200, San Antonio, TX 78245, USA. hornsby@uthscsa.edu <hornsby@uthscsa.edu> |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Cortex
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cytology,
physiology,
transplantation Aging / genetics*, physiology Animals Cattle Cell Transplantation* Humans Hydrocortisone / biosynthesis Mice Mice, SCID Models, Biological |
| Grant Support | |
ID/Acronym/Agency:
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AG 12287/AG/NIA NIH HHS; AG 20752/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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50-23-7/Hydrocortisone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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