| Using Pseudomonas putida xylE gene to teach molecular cloning techniques for undergraduates. | |
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MedLine Citation:
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PMID: 21567768 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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We have developed and implemented a serial experiment in molecular cloning laboratory course for undergraduate students majored in biotechnology. Pseudomonas putida xylE gene, encoding catechol 2, 3-dioxygenase, was manipulated to learn molecular biology techniques. The integration of cloning, expression, and enzyme assay gave students a chance to mimic real research and product development by connecting separated techniques. This serial experiment helped students set up intact idea of molecular biology and to understand the relation of each individual operation. |
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Authors:
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Xu Dong; Yi Xin; Li Ye; Yufang Ma |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology Volume: 37 ISSN: 1539-3429 ISO Abbreviation: Biochem Mol Biol Educ Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2011-05-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100970605 Medline TA: Biochem Mol Biol Educ Country: United States |
Other Details:
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Languages: eng Pagination: 339-43 Citation Subset: - |
Copyright Information:
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Copyright © 2009 International Union of Biochemistry and Molecular Biology, Inc. |
Affiliation:
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Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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