| Using Lactococcus lactis for glutathione overproduction. | |
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MedLine Citation:
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PMID: 15490155 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glutathione and gamma-glutamylcysteine were produced in Lactococcus lactis using a controlled expression system and the genes gshA and gshB from Escherichia coli encoding the enzymes gamma-glutamylcysteine synthetase and glutathione synthetase. High levels of gamma-glutamylcysteine were found in strains growing on chemically defined medium and expressing either gshA alone or both gshA and gshB. As anticipated, glutathione was found in a strain expressing gshA and gshB. The level of glutathione production could be increased by addition of the precursor amino acid cysteine to the medium. The addition of cysteine led to an increased activity of glutathione synthetase, which is remarkable because the amino acid is not a substrate of this enzyme. The final intracellular glutathione concentration attained was 358 nmol mg(-1) protein, which is the highest concentration reported for a bacterium, demonstrating the suitability of engineered L. lactis for fine-chemical production and as a model for studies of the impact of glutathione on flavour formation and other properties of food. |
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Authors:
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Yin Li; Jeroen Hugenholtz; Wilbert Sybesma; Tjakko Abee; Douwe Molenaar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-10-13 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 67 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-31 Completed Date: 2005-09-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 83-90 Citation Subset: IM |
Affiliation:
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Wageningen Centre for Food Sciences, NIZO food research, P.O. Box 20, 6710 Ede, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cloning, Molecular Culture Media / chemistry Cysteine / metabolism Cytosol / chemistry Dipeptides / biosynthesis Escherichia coli / enzymology, genetics Gene Expression Regulation, Bacterial Genes, Bacterial Glutamate-Cysteine Ligase / genetics, metabolism Glutathione / biosynthesis* Glutathione Synthase / genetics, metabolism Lactococcus lactis / genetics, metabolism* Oxidative Stress |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Dipeptides; 52-90-4/Cysteine; 636-58-8/gamma-glutamylcysteine; 70-18-8/Glutathione; EC 6.3.2.2/Glutamate-Cysteine Ligase; EC 6.3.2.3/Glutathione Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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