Document Detail


Using a fiber optic particle plasmon resonance biosensor to determine kinetic constants of antigen-antibody binding reaction.
MedLine Citation:
PMID:  23186304     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, one simple and label-free biosensing method has been developed for determining the binding kinetic constants of antiovalbumin antibody (anti-OVA) and anti-mouse IgG antibody using the fiber optic particle plasmon resonance (FOPPR) biosensor. The FOPPR sensor is based on gold-nanoparticle-modified optical fiber, where the gold nanoparticle surface has been modified by a mixed self-assembled monolayer for conjugation of a molecular probe reporter (ovalbumin or mouse IgG) to dock with the corresponding analyte species such as anti-OVA or anti-mouse IgG. The binding process, occurring when an analyte reacts with a probe molecule immobilized on the optical fiber, can be monitored in real-time. In addition, by assuming a Langmuir-type adsorption isotherm to measure the initial binding rate, the quantitative determination of binding kinetic constants, the association and dissociation rate constants, yields k(a) of (7.21 ± 0.4) × 10(3) M(-1) s(-1) and k(d) of (2.97 ± 0.1) × 10(-3) s(-1) for OVA/anti-OVA and k(a) of (1.45 ± 0.2) × 10(6) M(-1) s(-1) and k(d) of (2.97 ± 0.6) × 10(-2) s(-1) for mouse IgG/anti-mouse IgG. We demonstrate that the FOPPR biosensor can study real-time biomolecular interactions.
Authors:
Ting-Chou Chang; Chao-Ching Wu; Shau-Chun Wang; Lai-Kwan Chau; Wen-Hsin Hsieh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-11
Journal Detail:
Title:  Analytical chemistry     Volume:  85     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-11-13     Revised Date:  2013-12-18    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  245-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Immobilized / chemistry,  immunology
Antibody Affinity
Antigen-Antibody Reactions
Biosensing Techniques*
Gold / chemistry
Immunoglobulin G / chemistry,  immunology*
Kinetics
Metal Nanoparticles / chemistry
Mice
Optical Fibers
Ovalbumin / immunology*
Surface Plasmon Resonance*
Chemical
Reg. No./Substance:
0/Antibodies, Immobilized; 0/Immunoglobulin G; 7440-57-5/Gold; 9006-59-1/Ovalbumin
Comments/Corrections
Erratum In:
Anal Chem. 2013 Nov 5;85(21):10625

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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