Document Detail

Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae.
MedLine Citation:
PMID:  20153176     Owner:  NLM     Status:  MEDLINE    
In this study Fourier transform infrared micro-spectroscopy (FTIR) was used to determine lipid and carbohydrate content over time in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus subspicatus grown in batch culture in limiting concentrations of nitrogen (N). Both algae exhibited restricted cell division and increased cell size following N-limitation. FTIR spectra of cells in N-limited media showed increasing lipid:amide I and carbohydrate:amide I ratios over time. The use of lipid- and starch-staining dyes confirmed that the observed ratio changes were due to increased lipid and carbohydrate synthesis. These results demonstrate rapid metabolic responses of C. reinhardtii and S. subspicatus to changing nutrient availability, and indicate the efficiency of FTIR as a reliable method for high-throughput determination of lipid induction.
Andrew P Dean; David C Sigee; Beatriz Estrada; Jon K Pittman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-11
Journal Detail:
Title:  Bioresource technology     Volume:  101     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  4499-507     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Ltd. All rights reserved.
Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
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MeSH Terms
Algae / cytology,  drug effects*,  growth & development,  metabolism*
Amides / analysis
Carbohydrates / analysis
Cell Count
Cell Size
Chlamydomonas reinhardtii / cytology,  drug effects,  metabolism
Chlorophyll / metabolism
Culture Media / chemistry
Fresh Water*
Lipids / analysis*
Nitrogen / pharmacology*
Phosphorus / analysis
Principal Component Analysis
Scenedesmus / cytology,  drug effects,  metabolism
Spectroscopy, Fourier Transform Infrared
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Amides; 0/Carbohydrates; 0/Culture Media; 0/Lipids; 1406-65-1/Chlorophyll; 7723-14-0/Phosphorus; 7727-37-9/Nitrogen

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