Document Detail


Using FRET to Study RanGTP Gradients in Live Mouse Oocytes.
MedLine Citation:
PMID:  23138947     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Oocytes are extremely large cells that have to coordinate accurate chromosome segregation, asymmetric cytoplasm partitioning together with their own development as fertilizable gametes. For this, they undergo both global (cell cycle progression related) and local changes. It is therefore essential to be able to monitor local changes as they take place in live maturing oocytes. We describe here a method to follow RanGTP gradients using FRET technology in vivo.
Authors:
Julien Dumont; Marie-Hélène Verlhac
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  107-20     Citation Subset:  IM    
Affiliation:
Institut Jacques Monod, CNRS, UMR 7592, Univ Paris Diderot, Sorbonne Paris Cité, Paris, France, dumont@ijm.univ-paris-diderot.fr.
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