Document Detail


Using ENU mutagenesis for phenotype-driven analysis of the mouse.
MedLine Citation:
PMID:  20699149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of mutagenesis in invertebrates to generate phenotypic variants has a long and productive history. Despite the conclusive demonstration by Russell and colleagues in the 1970s that the chemical N-ethyl-N-nitrosourea (ENU) is a highly effective mutagen in mice, the application of phenotypic-driven mutagenesis as a method to study mammalian biology proceeded slowly. With the development of tools for genomic analysis, the task of positional cloning ENU-induced mutations has become quite feasible, and this approach has recently been widely applied and highly productive. It has specifically lived up to its theoretical utility as means to provide insight into the biological roles of genes that is not biased by presumptions of their function. While the power of this approach is indisputable, the effort necessary for its success remains substantial, requiring careful attention to aspects including ENU treatment, mouse husbandry, screen assay design, genetic mapping, positional cloning, and mutation validation. In this chapter we discuss practical aspects of implementing a phenotype-driven analysis of an ENU-mutagenized mouse population.
Authors:
Rolf W Stottmann; David R Beier
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  477     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-11     Completed Date:  2010-12-10     Revised Date:  2013-04-24    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  329-48     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Affiliation:
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ethylnitrosourea / pharmacology*
Mice
Mutagenesis / drug effects,  genetics*
Mutagens / pharmacology*
Phenotype
Grant Support
ID/Acronym/Agency:
R01 HD036404/HD/NICHD NIH HHS; R01 MH081187/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Mutagens; 759-73-9/Ethylnitrosourea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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