Document Detail

Using ENU mutagenesis for phenotype-driven analysis of the mouse.
MedLine Citation:
PMID:  20699149     Owner:  NLM     Status:  MEDLINE    
The use of mutagenesis in invertebrates to generate phenotypic variants has a long and productive history. Despite the conclusive demonstration by Russell and colleagues in the 1970s that the chemical N-ethyl-N-nitrosourea (ENU) is a highly effective mutagen in mice, the application of phenotypic-driven mutagenesis as a method to study mammalian biology proceeded slowly. With the development of tools for genomic analysis, the task of positional cloning ENU-induced mutations has become quite feasible, and this approach has recently been widely applied and highly productive. It has specifically lived up to its theoretical utility as means to provide insight into the biological roles of genes that is not biased by presumptions of their function. While the power of this approach is indisputable, the effort necessary for its success remains substantial, requiring careful attention to aspects including ENU treatment, mouse husbandry, screen assay design, genetic mapping, positional cloning, and mutation validation. In this chapter we discuss practical aspects of implementing a phenotype-driven analysis of an ENU-mutagenized mouse population.
Rolf W Stottmann; David R Beier
Related Documents :
19924099 - Genetic analysis of albuminuria in aging mice and concordance with loci for human diabe...
4015839 - Biochemical and genetic studies on mouse aldehyde dehydrogenases.
21045809 - Novel properties of tyrosine-mutant aav2 vectors in the mouse retina.
19869379 - Studies on the mode of spread of b. enteritidis mouse typhoid infection : ii. effects o...
19897589 - Overexpression of hr links excessive induction of wnt signaling to marie unna hereditar...
1498039 - Disorganization is a completely dominant gain-of-function mouse mutation causing sporad...
21179519 - New microsatellite markers for examining genetic variation in peripheral and core popul...
14755409 - Radiographic and genetic diagnosis of sporadic hypochondroplasia early in the neonatal ...
20196079 - Oncogenic kras is not necessary for wnt signalling activation in apc-associated fap ade...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  477     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-11     Completed Date:  2010-12-10     Revised Date:  2013-04-24    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  329-48     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Ethylnitrosourea / pharmacology*
Mutagenesis / drug effects,  genetics*
Mutagens / pharmacology*
Grant Support
Reg. No./Substance:
0/Mutagens; 759-73-9/Ethylnitrosourea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Phenotype-driven mouse ENU mutagenesis screens.
Next Document:  Exploration of self-renewal and pluripotency in ES cells using RNAi.