Document Detail


Using Cell-ID 1.4 with R for microscope-based cytometry.
MedLine Citation:
PMID:  23026908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This unit describes a method for quantifying various cellular features (e.g., volume, total and subcellular fluorescence localization) from sets of microscope images of individual cells. It includes procedures for tracking cells over time. One purposely defocused transmission image (sometimes referred to as bright-field or BF) is acquired to segment the image and locate each cell. Fluorescence images (one for each of the color channels to be analyzed) are then acquired by conventional wide-field epifluorescence or confocal microscopy. This method uses the image-processing capabilities of Cell-ID and data analysis by the statistical programming framework R, which is supplemented with a package of routines for analyzing Cell-ID output. Both Cell-ID and the analysis package are open-source.
Authors:
Alan Bush; Ariel Chernomoretz; Richard Yu; Andrew Gordon; Alejandro Colman-Lerner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]     Volume:  Chapter 14     ISSN:  1934-3647     ISO Abbreviation:  Curr Protoc Mol Biol     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-01-30     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  8908160     Medline TA:  Curr Protoc Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 14.18     Citation Subset:  IM    
Copyright Information:
2012 by John Wiley & Sons, Inc.
Affiliation:
IFIByNE-CONICET and Department of Physiology, Molecular and Cellular Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Cell Tracking / methods*
Computational Biology / instrumentation,  methods
Fluorescence Resonance Energy Transfer / methods
Image Cytometry / methods*
Image Processing, Computer-Assisted / instrumentation,  methods*
Microscopy, Fluorescence / instrumentation,  methods*
Grant Support
ID/Acronym/Agency:
0000713502//PHS HHS; 1R01GM097479-01/GM/NIGMS NIH HHS; R01 GM097479/GM/NIGMS NIH HHS
Comments/Corrections

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