Document Detail

Using ABCG2-molecule-expressing side population cells to identify cancer stem-like cells in a human ovarian cell line.
MedLine Citation:
PMID:  21108606     Owner:  NLM     Status:  MEDLINE    
CSCs (cancer stem cells) are a small subset of cells within a tumour that possesses the characteristics of stem cells and are considered to be responsible for resistance to chemoradiation. Identification of CSCs through stem cell characteristics might have relevant clinical implications. In this study, SP (side population ) cells were sorted from a human ovarian cancer cell line by FACS to determine whether cancer stem cell-like SP cells were present. A very small fraction of SP cells (2.6%) was detected in A2780 cells. SP cells possessed the following characteristics: highly proliferative activity, marked ability for self-renewal in soft agar and culture medium, high expression of ABCG2, drug resistance to vinblastine in vitro, and strong tumourigenic potential in Balb/c nude mice. It is concluded that there exists in the A2780 cell line a small number of SP cells with high expression of ABCG2. The cells have the characteristics of cancer stem-like cells, and identification and cloning of such human SP cells can help in improving therapeutic approaches to ovarian cancer in patients.
Jun Dou; Cuilian Jiang; Jing Wang; Xian Zhang; Fengshu Zhao; Weihua Hu; Xiangfeng He; Xiaoli Li; Dandan Zou; Ning Gu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-11     Completed Date:  2011-06-10     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  227-34     Citation Subset:  IM    
Department of Pathogenic Biology and Immunology, Southeast University Medical School, Nanjing, Peoples Republic of China.
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MeSH Terms
ATP-Binding Cassette Transporters / metabolism*
Antineoplastic Agents, Phytogenic / therapeutic use
Cell Line, Tumor
Cell Proliferation
Cell Separation
Drug Resistance, Neoplasm
Mice, Inbred BALB C
Mice, Nude
Neoplasm Proteins / metabolism*
Neoplastic Stem Cells / cytology,  metabolism*
Ovarian Neoplasms / drug therapy,  metabolism*,  pathology
Side-Population Cells / cytology,  metabolism*
Vincristine / therapeutic use
Reg. No./Substance:
0/ABCG2 protein, human; 0/ATP-Binding Cassette Transporters; 0/Antineoplastic Agents, Phytogenic; 0/Neoplasm Proteins; 57-22-7/Vincristine

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