Document Detail


Uselessness of anti-actin antibody in celiac disease screening.
MedLine Citation:
PMID:  18252201     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Serum anti-actin IgA antibodies (AAA) were identified in patients with celiac disease (CD), and a close correlation emerged between the presence of AAA and mucosa damage, but test for AAA found in celiacs have a wide range of sensitivity and specificity values. AIM: To compare 1) the sensitivity and specificity of untreated, calcium-chelated and heated sera from 102 celiacs, 52 sick patients and 103 healthy controls in the determination of AAA, and 2) the reliability of AAA with anti-transglutaminase antibodies (anti-tTG) in diagnosing celiac disease and in predicting intestinal damage. The intestinal derived AAA was isolated by using the phage-display library technique. RESULTS: Treated sera was significantly more sensitive than untreated (p=0.0001), and showed a significant correlation between AAA and the three degrees (3a, 3b, 3c) of intestinal damage (p=0.01). Sensitivity and specificity values of anti-tTG assay were higher than the AAA assay, and anti-tTG serum-concentration was only significantly correlated with more severe (3b and 3c) intestinal damage degrees. AAA isolated by phage display showed similar results of serum AAA in immunofluorescence assay. CONCLUSIONS: Notwithstanding correlation between AAA and celiac disease, AAA assay, also after treatments, has little to offer in screening for CD compared to the well-established anti-transglutaminase assay.
Authors:
Elisa Fabbro; Laura Rubert; Sara Quaglia; Fortunato Ferrara; Valentina Kiren; Alessandro Ventura; Tarcisio Not
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-24
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  390     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-03     Completed Date:  2008-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  134-7     Citation Subset:  IM    
Affiliation:
Department of Reproductive and Development Science, University of Trieste and I.R.C.C.S. "Burlo Garofolo" Trieste, Italy.
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MeSH Terms
Descriptor/Qualifier:
Actins / immunology*
Adolescent
Adult
Autoantibodies / analysis*
Celiac Disease / diagnosis*,  immunology
Cell Line, Tumor
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Humans
Male
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Actins; 0/Autoantibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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