| Uselessness of anti-actin antibody in celiac disease screening. | |
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MedLine Citation:
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PMID: 18252201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Serum anti-actin IgA antibodies (AAA) were identified in patients with celiac disease (CD), and a close correlation emerged between the presence of AAA and mucosa damage, but test for AAA found in celiacs have a wide range of sensitivity and specificity values. AIM: To compare 1) the sensitivity and specificity of untreated, calcium-chelated and heated sera from 102 celiacs, 52 sick patients and 103 healthy controls in the determination of AAA, and 2) the reliability of AAA with anti-transglutaminase antibodies (anti-tTG) in diagnosing celiac disease and in predicting intestinal damage. The intestinal derived AAA was isolated by using the phage-display library technique. RESULTS: Treated sera was significantly more sensitive than untreated (p=0.0001), and showed a significant correlation between AAA and the three degrees (3a, 3b, 3c) of intestinal damage (p=0.01). Sensitivity and specificity values of anti-tTG assay were higher than the AAA assay, and anti-tTG serum-concentration was only significantly correlated with more severe (3b and 3c) intestinal damage degrees. AAA isolated by phage display showed similar results of serum AAA in immunofluorescence assay. CONCLUSIONS: Notwithstanding correlation between AAA and celiac disease, AAA assay, also after treatments, has little to offer in screening for CD compared to the well-established anti-transglutaminase assay. |
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Authors:
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Elisa Fabbro; Laura Rubert; Sara Quaglia; Fortunato Ferrara; Valentina Kiren; Alessandro Ventura; Tarcisio Not |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-24 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 390 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-03 Completed Date: 2008-06-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 134-7 Citation Subset: IM |
Affiliation:
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Department of Reproductive and Development Science, University of Trieste and I.R.C.C.S. "Burlo Garofolo" Trieste, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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immunology* Adolescent Adult Autoantibodies / analysis* Celiac Disease / diagnosis*, immunology Cell Line, Tumor Child Child, Preschool Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique, Indirect Humans Male Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Autoantibodies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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