| Usefulness of specific OA biomarkers, thrombin-cleaved osteopontin, in the posterior cruciate ligament OA rabbit model. | |
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MedLine Citation:
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PMID: 23025929 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: We undertook this study to determine whether thrombin-cleaved osteopontin (OPN) in synovial fluid (SF) represent a useful marker of osteoarthritis (OA) progression in the posterior cruciate ligament transection (PCLT) OA rabbit model. METHOD: PCLT was performed on the right knee joints of 48 rabbits. The rabbits were then sacrificed separately at 4, 8, 16, and 24 weeks post-surgery, when the joint was harvested and macroscopic and histological assessments of articular cartilage were performed. Thrombin-cleaved OPN product in SF was determined using western blotting and the levels were measured using an enzyme-linked immunoassay. RESULTS: The macroscopic and histological scores for PCLT knees were already elevated 4 weeks after surgery and increased with time. Western blotting showed the presence of thrombin-cleaved OPN in SF from PCLT knees. Thrombin-cleaved OPN levels in SF were elevated at 4 weeks (P < 0.001) and were elevated peaking at 24 weeks (P < 0.00001) after PCLT compared to baseline. A positive significant correlation was found between thrombin-cleaved OPN levels and the macroscopic scores (8 weeks: ρ=0.695, p=0.012; 16 weeks: ρ=0.751, p=0.005; 24 weeks: ρ=0.660, p=0.020). Furthermore, the same correlation was noted between thrombin-cleaved OPN levels and the histological scores (4 weeks: ρ=0.609, p=0.036; 8 weeks: ρ=0.662, p=0.019; 16 weeks: ρ=0.827, p=0.001; 24 weeks: ρ=0.813, p=0.001). CONCLUSION: In this rabbit model of PCLT, thrombin-cleaved OPN levels in SF appear to provide a useful marker of OA disease severity and progression. |
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Authors:
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Shu-Guang Gao; Ling Cheng; Chao Zeng; Li-Cheng Wei; Fang-Jie Zhang; Jian Tian; Min Tu; Wei Luo; Guang-Hua Lei |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-28 |
Journal Detail:
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Title: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society Volume: - ISSN: 1522-9653 ISO Abbreviation: Osteoarthr. Cartil. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-10-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9305697 Medline TA: Osteoarthritis Cartilage Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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Department of Orthopaedics, Xiangya Hospital, Central South University, #87 Xiangya Road, Changsha, Hunan, China 410008. Electronic address: shuguang.gao@gmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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