| Usefulness of real-time PCR in long-term follow-up of follicular lymphoma patients. | |
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MedLine Citation:
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PMID: 17351675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to evaluate the usefulness of quantitative real-time PCR (RQ-PCR) for the monitoring of molecular remission in follicular lymphoma (FL) patients during long-term follow-up. RQ-PCR by the use of TaqMan detection system is a sensitive tool to monitor minimal residual disease (MRD) in FL through amplification of the t(14;18) fusion gene during and post-therapy. In most cases the breakpoint region occurs within the major breakpoint region (MBR). Among 75 patients diagnosed with FL, cells harboring the fusion gene BCL2/JH were found in peripheral blood of 31 patients (41%). We further monitored 30 of these patients in a period varying from 6 months to 5 years by RQ-PCR. In our study the level indicating the possibility of the presence of MRD was established at more than five t(14;18)-positive cells in the background of 83,000 normal cells. The results of this work also confirmed that the presence of MRD detected by RQ-PCR is an indication for careful observation of patients because of a higher risk of disease recurrence. |
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Authors:
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Andrzej Tysarowski; Anna Fabisiewicz; Ewa Paszkiewicz-Kozik; Jadwiga Kulik; Jan Walewski; Janusz A Siedlecki |
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Publication Detail:
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Type: Journal Article Date: 2007-03-09 |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 54 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-03-27 Completed Date: 2007-10-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: Poland |
Other Details:
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Languages: eng Pagination: 135-42 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warszawa, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Antineoplastic Combined Chemotherapy Protocols / therapeutic use Chromosomes, Human, Pair 14 Chromosomes, Human, Pair 18 Female Follow-Up Studies Humans Lymphoma, Follicular / classification, drug therapy, genetics*, pathology Male Middle Aged Polymerase Chain Reaction / methods* Translocation, Genetic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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